Table 1.
BV2 microglia in three-dimensional (3D) cultures show increased expression of IL-6, TNF-α and MCP-1 at 48 hours post-lipopolysaccharide (LPS) stimulation
| |
Untreated |
LPS-treated |
||||
|---|---|---|---|---|---|---|
| IL-6 | TNF-α | MCP-1 | IL-6 | TNF-α | MCP-1 | |
|
Monolayer |
1.7 ± 0.3 |
252.3 ± 120.5 |
9 447.0 ± 226.8 |
2 476.0 ± 833.6** |
3 823.0 ± 1189.0** |
10 459.0 ± 1, 639.0ns |
|
Coated monolayer |
1.6 ± 0.99 |
217.6 ± 181.8 |
9 231.0 ± 2268.0 |
1 764.0 ± 324.3** |
2 357.0 ± 500.9** |
10 936.0 ± 1292.0ns |
| 3D | 0.7 ± 0.8 | 8.1 ± 3.1 | 284.0 ± 73.5 | 1 999.0 ± 685.2* | 1 744.0 ± 911.6* | 5 403.0 ± 517.6* |
Values are expressed in pg/ml, and assayed using the Cytometric Bead Array. Data are mean ± SD from five independent experiments. *p < .05, **p < .01; two-tailed Mann-Whitney U test, as compared to untreated controls. ns, not significant.