Figure 5.
The loss of VGluT1+ neuronal glutamatergic synaptic activity significantly decreases developmental induction of GLT1 in vivo. A, Representative GLT1 immunoblot in sensory-motor cortex of VGluT1−/−, VGluT1+/−, and VGluT1+/+ mice at P14. B, GLT1 mRNA expression levels in sensory-motor cortex of VGluT1−/− and VGluT1+/+ mice at P26. N = 4 mice/group; p = 0.019 as determined using the Student's t test. C, D, Representative GLT1 immunoblot (C) and quantification of GLT1 protein expression levels (D) in sensory-motor cortex of VGluT1−/−, VGluT1+/−, and VGluT1+/+ mice at P26. N = 3–4 mice/group. *p < 0.05; **p < 0.01 as determined in one-way ANOVA analysis with Tukey's multiple-comparisons test. E, F, Expression levels of GLAST (E) and EAAC1 (F) in sensory-motor cortex of VGluT1−/− and VGluT1+/+ mice at P26. N = 4 mice/group. p value was determined using the Student's t test;.