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. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: Semin Liver Dis. 2009 Apr 22;29(2):178–187. doi: 10.1055/s-0029-1214373

“Second Hit” Models of Alcoholic Liver Disease

Hidekazu Tsukamoto 1,2,4, Keigo Machida 1,3, Alla Dynnyk 1,2, Hasmik Mkrtchyan 1,2
PMCID: PMC4197797  NIHMSID: NIHMS629346  PMID: 19387917

Abstract

Alcoholic liver disease (ALD) is a lifestyle disease with its pathogenesis and individual predisposition governed by gene–environment interactions. Based on the “second hit” or “multiple hits” hypothesis, patients are predisposed to progressive ALD when a magic combination of gene and environmental interactions exists. Reproduction of second or multiple hits in animal models serves to test a combination and to gain mechanistic insights into synergism achieved by such combination. Numerous environmental factors have been incorporated into animal models, largely classified into nutritional, xenobiotic/pharmacologic, hemodynamic, and viral groups. A loss or gain of function genetic model has become a popular experimental approach to test the role of a gene as a second hit. Future research will need to test more subtle or natural hits combined with excessive alcohol intake to test multiple hits in the genesis of ALD. Additionally, animal models of comorbidities are urgently needed particularly for synergistic liver disease and oncogenesis caused by alcohol, obesity, and hepatitis virus.

Keywords: Gene-environment interactions, alcoholic liver disease, alcoholic steatohepatitis, nonalcoholic steatohepatitis, nonalcoholic fatty liver disease, animal models

THE SECOND HIT HYPOTHESIS

Alcoholic and nonalcoholic steatohepatitis (ASH and NASH) are the two most common lifestyle liver diseases worldwide caused by excessive intake of alcohol and calories, respectively. Pathological evolution of ASH and NASH are very similar if not identical. A majority of alcoholic or obese patients develop hepatic steatosis, but progression to steatohepatitis occurs only in 15 to 20% of the patients.1,2 Although ASH and NASH are considered as critical turning points for the increased propensity for progression to cirrhosis, a fraction (20 to 50%) of ASH and NASH patients develop cirrhosis.1,2 The fact that most alcoholic or obese patients develop fatty liver, but experience a sharp decline in the incidence of ASH/NASH and cirrhosis, has led to a proposal that excessive intake of alcohol or calories is the first hit to induce hepatic steatosis, the early stage of alcoholic liver disease (ALD), or nonalcoholic fatty liver disease (NAFLD). The second hit is required for the development of ASH or NASH and of cirrhosis (Fig. 1). The obvious and important question is what this second hit is. Indeed, this is the question addressed by many investigators, which has resulted in the generation of numerous second hit ASH or NASH animal models summarized below. However, it is very important to recognize that the second hit question cannot be discussed without understanding the gene–environment interactions that govern the pathogenesis of most chronic diseases of various organs including the liver. Most likely, distinct groups of genes participate in the pathogenesis of different stages of the ASH/NASH pathologic spectrum, and environmental factors interplay with suspected genes for their expression and participation in the pathogenesis (Fig. 2). There also exist cross-interactions within the genes as well as within environmental factors. These complex interactions between and among genetic and environmental factors must determine the difference in individual predisposition to progression of ASH and NASH. From this view, the second hit hypothesis may be too simplistic, and the insults may most likely be “multiple” (Fig. 1). Indeed, the more risk factors an individual has, the more predisposed she or he would be for progressive ASH and NASH. Thus, for the development of ASH or NASH animal models, both genetic and environmental factors need to be considered, and those which achieve most synergistic interactions are likely most successful in reproducing progressive pathology. Once such synergism is reproduced, the animal model serves to help identify its molecular mechanisms and new therapeutic targets for the disease.

Figure 1.

Figure 1

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The “second hit” hypothesis of alcoholic steatohepatitis (ASH) and nonalcoholic steatohepatitis (NASH) pathogenesis. The second hit is most likely to be multiple hits in progression of ASH and NASH from hepatic steatosis.

Figure 2.

Figure 2

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Gene–environment interactions that determine the individual susceptibility to alcoholic liver disease.

SECOND HIT ANIMAL MODELS FOR ALD

Table 1 summarizes a partial list of second hit animal models for alcoholic liver injury. As one may quickly realize, the listed factors and genes are known players or regulators in the pathogenesis of ALD. How some of these second hits mechanistically fit into the known mechanisms of the ALD pathogenesis is depicted by shaded boxes and bold arrows in Fig. 3. Second hit models are categorically divided into nutritional, ago-nistic/xenobiotic/pharmacologic, hemodynamic, hepatitis C virus (HCV) and genetic groups in Table 1, and are briefly reviewed with scientific backgrounds below.

Table 1.

“Second Hit” Alcoholic Liver Disease Animal Models

Reference
Nutritional
    High fat diet 4,80
    Fish oil 12,13
    Lron 19,22
    Folate deficiency 26
    Choline deficiency 27
Agonistic/Xenobiotic/Pharmacologic
    TLR ligands (e.g., LPS) 36,38,81
    Concavalin A 39
    Aldehyde adducts (acetaldehyde, MAA) 40–43
    Carbon tetrachloride 45,46
    Pyrazole 44
    Acetaminophen 49
    Epinephrine 50
    Thyroid hormone 51
Hemodynamic
    Ischemia/reperfusion 57
    Hypoxia 58
HCV
    Core 64
    NS5A 65
Genetic
    Female sex 71
    CYP2E1 Tg 66
    Nrf2−/− 67
    CuZnSOD−/− 68
    IL-6−/− 69
    hepSTAT3−/− 79
    fa/fa 7
    CBS+/− 70
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TLR, toll-like receptor; LPS, lipopolysaccharide; MAA, malondialdehyde-acetaldehyde adducts; CYP2E1, cytochrome P450 2E1; CuZn-SOD, copper zinc superoxide dismutase; hepSTAT3, hepatocyte specific knockout of signal transducer and activator of transcription 3; CBS, cystathionine β synthase.

Figure 3.

Figure 3

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How diverse “second hits” interplay with known pathogenetic mechanisms of alcoholic liver disease. Shaded boxes and bold arrows depict some of the second hits discussed in this review.

Nutritional Second Hit

The most common environmental second hit approach is nutritional modification. High fat diet (HFD) has long been known to promote alcoholic liver injury in rodents. Among many effects possibly caused by HFD, the most notable is induction of cytochrome P450 2E1 (CYP2E1).3 As isocaloric feeding is usually employed, a high level of fat results in a reciprocally lower carbohydrate content, and this low carbohydrate-HFD induces CYP2E1, and consequent oxidant stress, and alcoholic liver damage.4–6 HFD also causes obesity, insulin resistance (IR), and NAFLD. Thus, this second hit may also reflect synergistic hepatic injury associated with dual effects of alcohol and obesity/IR. Further, this synergism may also be linked to CYP2E1 as over-expression of this cytochrome p450 isozyme induces hepatic IR.8 Another potential mechanism induced by HFD is endoplasmic reticulum (ER) stress also described as an unfolded protein response.9,10 ER stress is implicated in ALD,11 and HFD may worsen this condition via its ability to promote ER stress, which, in turn, induces IR.9 The type of fat is also an important consideration, and polyunsaturated fat is implicated in many of the aforementioned HFD effects. Fish oil (n-3 polyunsaturated fatty acids), in particular, is shown to aggravate experimental ALD.12,13 However, fish oil activates peroxisome proliferator–activated receptor alpha (PPARα) and suppresses de novo lipogenesis in the mouse model of steatohepatitis.14 Pretreatment with fish oil even attenuates fatty liver induced by an acute dose of ethanol, the effect which is associated with PPARα activation and reduced sterol regulatory element binding protein-1c (SREBP-1c) expression.15 These contradictory effects may be attributable to the fact that fish oil increases hepatic levels of lipid peroxides14; this effect in the setting of chronic alcohol intake may override the beneficial effects and exacerbates experimental ALD.

Iron as a Second Hit

Interactions between iron and ALD have long been suggested for chronic liver disease including ALD.16 A consensus has been that iron accumulates in chronic liver inflammation and, in turn, catalyzes hydroxyl radical-mediated oxidant damage via the Fenton pathway. In fact, the treatment with an iron chelator attenuates alcoholic17 and cholestatic18 liver injury in animal models. Conversely, iron supplementation with carbonyl iron, accelerates alcoholic liver damage,19 and this iron loading can be an important second hit. The carbonyl iron method primarily achieves iron loading in hepatocytes where iron catalyzes and promotes oxidant stress and damage. However, iron also accumulates in Kupffer cells in ALD20 and NASH21 models. This iron accumulation leads to an expansion of a chelatable pool of iron, which, in turn, augments lipopolysaccharide-(LPS-) or peroxynitrite-induced iron signaling in Kupffer cells to accentuate IkappaB kinase (IKK) and nuclear factor kappa B (NFκB) activation.20,22 This novel iron signaling mechanism can be exploited to achieve a second hit. Iron dextran, if injected subcutaneously, is gradually and selectively taken up by macrophages, and this should theoretically increase iron content in macrophages including Kupffer cells and promote proinflammatory NFκB activation. Indeed, this actually happens. Kupffer cells, isolated from a normal mouse given a single dose of iron dextran, release 4- to 5-fold more tumor necrosis factor-α (TNFα) in response to LPS or peroxynitrite (Fig. 4A). This treatment in the mouse intragastric ethanol infusion model markedly exacerbates alcoholic liver injury (Fig. 4B – D). Immunostaining demonstrates localization of active p65 in hepatic macrophages (Fig. 4F) loaded with iron (Fig. 4E). This treatment even promotes liver fibro-genesis within only 4 weeks of alcohol feeding as depicted by reticulin staining (Fig. 4G) and α-smooth muscle actin staining, which identifies activated hepatic stellate cells (Fig. 4H).

Figure 4.

Figure 4

Figure 4

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(A) Kupffer cells (KC) were isolated from mice at 2 weeks after a single subcutaneous injection of iron dextran (25 mg/kg) to assess lipopolysaccharide (LPS) or peroxynitrite stimulated tumor necrosis factor-α (TNFα) expression. Note KC from iron dextran-injected mice release 4 to 5 times more TNFα in response to LPS or peroxynitrite. p < 0.05 compared with the cells from mice without iron dextran injection. (B) Plasma alanine aminotransferase (ALT) levels of mice fed intragastrically with alcohol and high fat diet or pair-fed with isocaloric diet for 4 weeks without or with prior iron dextran injection. Note a single subcutaneous injection of iron dextran (75 mg/kg) prior to alcohol feeding significantly aggravates plasma ALT elevation as compared with alcohol-fed mice injected with vehicle (dextran). (C) Liver histological score of mice fed alcohol for 4 weeks without or with iron dextran injection. Note iron dextran injection at 75 mg/kg significantly worsens the liver histologic score compared with alcohol-fed mice with vehicle treatment. (D) Morphometric analysis of inflammatory cells in the liver of the different experimental groups. Note iron dextran injection significantly increases inflammatory cells in the liver as compared with alcohol-fed mice with vehicle treatment. (E) Representative microphotographs of aggravated alcoholic liver injury in iron dextran-injected mice. In the liver of alcohol-fed mice given 75 mg/kg iron dextran, hepatic macrophages stained for iron (Prussian blue reaction) are noted. (F) These iron staining-positive macrophages are also stained positively for active p65. (G) Pericellular liver fibrosis is evident in the livers of alcohol-fed mice with iron dextran treatment as demonstrated by reticulin staining. (H) Numerous activated hepatic stellate cells are present in a focus of liver necrosis and inflammation as shown by α-smooth muscle actin staining. (From Xiong S, She H, Zhang AS, et al. Hepatic macrophage iron aggravates experimental alcoholic steatohepatitis. Am J Physiol Gastrointest Liver Physiol 2008;295(3):G512–G521. Reprinted with permission from The American Physiological Society.)

Other Nutritional Second Hit Models

Chronic alcohol intake causes the deficiency of micro-nutrients such as zinc,23 folate,24 and choline,25 to list a few. Combination of alcohol intake and a diet deficient in folate26 or choline27 aggravates experimental ALD, supporting the pathogenetic importance of the micronutrient deficiencies. These deficiencies converge, besides their other independent physiological complications, to abnormal methionine metabolism well documented in ALD.28,29 Folate is required along with vitamin B12 for methionine synthesis from homocysteine catalyzed by methionine synthase. Choline constitutes a polar head for phosphatidylcholine generated by methylation of phosphatidylethanolamine using S-adenosylmethionine (SAMe) as the methyl donor, the process likely suppressed by reduced SAMe in ALD. Choline is also a precursor for trimethylglycine (betaine), which is required for another methionine resynthesis pathway via betaine-homocysteine methyltransferase (BHMT). Betaine supplementation or BHMT expression attenuates homocystinemia, SAMe depletion, and experimental ALD,30–32 supporting the role of abnormal methionine metabolism in the ALD pathogenesis. A defect in any of the key components of methionine metabolism, may serve as a second hit for ALD. Such an example is loss of function of glycine N-methyltransferase (GNMT), which results in hepatic steatosis, fibrosis and cancer in mice.33 GNMT catalyzes methylation of glycine to form N-methylglycine and by doing so removes excess SAMe and maintains a physiologic ratio of SAMe to S-adeno-sylhomocysteine (SAH) for normal methylation status. GNMT is downregulated in patients with alcoholic or/and HCV-induced liver cirrhosis,34 and as much as 40% of hepatocellular carcinoma (HCC) patients show loss of heterozygosity for GNMT.35 A study with GNMT knockout mice reveals reduced hepatic expression of the Janus kinases (Jak)/signal transducers and activators of transcription (STAT) inhibitors suppressor of cyto-kine signaling (SOCS) 1–3 and the Ras inhibitors RASSF as potential mechanistic insights into enhanced oncogenesis.33

Agonistic/ Xenobiotic/ Pharmacologic Second Hit

One prominent feature of chronic alcohol intake is its ability to sensitize the liver for agonistic activation of pathogen-associated pattern recognition receptors such as toll-like receptors (TLRs). The most classical demonstration of this effect is induction of massive liver necrosis and inflammation by injection of the TLR4 ligand, LPS in ethanol-fed animals.36 Indeed, the path-ogenetic roles of gut-derived endotoxin, LPS binding protein, CD14, and Kupffer cell activation in experimental ALD, have been convincingly demonstrated by a series of studies with gut sterilization, Kupffer cell depletion, and genetic mice performed by Ron Thurman’s laboratory.37 Alcohol intake increases messenger ribonucleic acid (mRNA) expression of not only TLR4, but also TLR2 and TLR6, which are activated by peptidoglycan; TLR7 and TLR8, which recognize viral single-stranded RNA; and TLR9, which recognizes bacterial and viral unmethylated CpG-containing DNA.38 Accordingly, administration of the ligands to some of these receptors, particularly for TLR2/6 and TLR4, aggravates alcoholic liver injury in mice.38 Interestingly, this study also demonstrates synergistic liver damage in ethanol-fed mice with polyIC and flagellin, the ligands for TLR3 and TLR4, despite the fact that mRNA levels of these receptors and TNFα are not increased by alcohol feeding or/and ligand treatment.38 Alcohol also primes T cells and this priming results in concavalin A-induced hepatitis in alcohol-fed rats; this pathology is reproduced in control mice after adaptive transfer of T cells from alcohol-fed rats.39 This model deserves further investigation for mechanisms of liver T cell priming by alcohol feeding. Alcohol-induced adaptive immunity can also be a target for the second hit. This is exemplified by experimental hepatitis and liver fibrosis induced by administration of acetaldehyde adducts in ethanol-fed guinea pigs40,41 or proinflammatory and profibrogenic responses elicited by malondialdehyde-acetaldehyde adducts (MAA), more naturally occurring hybrid adducts in ALD.42,43

Induction of CYP2E1 or isozymes as discussed above in relation to HFD, can be achieved by xenobiotics such as carbon tetrachloride or pyrazole, which can serve as a second hit to promote alcohol or LPS-induced liver cell death and inflammation44 and to even induce liver fibrosis.45,46 Increased susceptibility of alcoholic patients to acetaminophen toxicity has long been recognized.47 This interaction may be due to induction of CYP2E1 by alcohol with more robust production of N-acetyl-p-quinoneimine, a toxic metabolite of acetaminophen, or more susceptible hepatic mitochondria with reduced glutathione in alcohol-consuming subjects, which are readily targeted by acetaminophen-mediated cell death signaling involving c-Jun N-terminal kinasen (JNK).48 A second hit model with acetaminophen has been reproduced in rats given repetitive alcohol binge intake.49

The hormones, epinephrine, and thyroxin can also render a second hit insult to aggravate experimental ALD in rodents.50,51 These manipulations have the scientific rationale that alcohol consumption results in hypermetabolic state of the liver mediated by catecholamines52 or thyroid hormones,53 which contributes to accentuated centrilobular hypoxia and injury in ALD. The effects of catecholamine may also cause induction of plasminogen activator inhibitor-1 (PAI-1), which favors procoagulative and proinflammatory conditions in experimental ALD54 as seen in patients with ALD.55

Hemodynamic Second Hit

As discussed above, chronic alcoholism results in increased hepatic oxygen consumption and consequent centrilobular hypoxia due to the hypermetabolic state and inadequate compensation by increased oxygen delivery via portal blood flow.56 Such liver is vulnerable to ischemia/reperfusion due to accentuated oxidant stress, NFκB activation, chemokine expression, and neutrophilic infiltration,57 or transient hypoxia because of adenosine triphosphate (ATP) depletion.58 The procoagulative condition, with induced PAI-1 as discussed above, may also contribute to microcirculation disturbance at the sinusoidal level and worsen hypoxic insult.

HCV Proteins as Second Hits

Compelling evidence exists for synergistic liver damage caused by alcohol and HCV, which culminates in an increased incidence of HCC. Recent studies with mice expressing HCV proteins have shed pivotal insights into the mechanisms underlying the synergism. The HCV core protein causes overproduction of reactive oxygen species, which appears to be responsible for mitochondrial DNA damage.59 The core protein also inhibits microsomal triglyceride transfer protein activity and very low density lipoprotein secretion,60 which may underlie the genesis of fatty liver. The core protein also induces IR in mice and cell lines. This effect may be mediated by degradation of insulin receptor substrates (IRSs) 1 and 2 via upregulation of SOCS3,61 in a manner dependent on PA28γ,62 or via IRS serine phosphorylation.63 Thus, these core-induced perturbations such as oxidant stress and IR, which are known risk factors for ALD, can explain the synergism reproduced in alcohol-fed core transgenic mice.64 A most recent study with mice that express one of the HCV’s nonstructural proteins, NS5A in a hepatocyte-specific manner, further demonstrates synergistic alcoholic liver damage and liver oncogenesis with this viral protein. These consequences are attributable to NS5A-induced TLR4, the pattern recognition receptor known to be indispensable for experimental ALD.65 Second hit models utilizing these genetic mouse models are expected to shed more molecular insights into the most important and clinically relevant pathologic consequences of synergism resulting from alcohol and HCV.

Genetic Second Hit

Genetic second hit models basically apply either a gain of function approach for a suspected causal gene such as CYP2E166 or a loss of function manipulation for a protective gene such as Nrf2, superoxide dismutase 1 (CuZnSOD), interleukin-6 (IL-6), or cystathionine β synthase (CBS)67–70 as examples. The former is intended to promote an injurious mechanism, and the latter approach sensitizes the liver for alcohol-mediated harmful effects. The best example of a genetic model that does not require any genetic manipulation is that of female sex.71 Using the intragastric ethanol infusion model, female rats were shown to exhibit more accelerated liver damage with more panlobular hepatic steatosis, heightened inflammation, and plasma aminotransferase levels.71 Why the female gender causes an increased susceptibility to ALD is an important and clinically relevant question. Based on studies with female animals, several potential mechanisms have been proposed to date. These include less gastric, first-pass metabolism,72 increased endotoxemia,73 higher responsiveness of Kupffer cells to endotoxin,74 more lysosomal leakage and less BHMT expression,75 and enhanced osteopontin expression.76

Nrf2 (NF-E2-related factor 2) is a basic leucine zipper transcription factor that binds to the antioxidant-responsive element to induce transcription of detoxification and antioxidant genes such as glutathione S-transferase, NAD(P)H:quinine oxidoreductase-1, glutamate-cysteine ligase, heme oxygenase-1, thioredoxin reductase 1, and glucose-6-phosphate dehydrogenase. Nrf2 deficiency aggravates acetaminophen hepatotoxicity77 and alcoholic liver injury.67 Oxidant stress and protein kinases activate cytoplasmic Nrf2 by disrupting Nrf2-Keap1 complex leading to nuclear translocation. Interestingly, PKR-like ER kinase (PERK), one of the ER stress effector kinases, also phosphorylates and activates Nrf2 to facilitate a survival response.78 Thus, oxidant stress and ER stress converge onto Nrf2, and regulation and/or polymorphism of this gene may underlie a different predisposition to ALD. Deficiency in CuZnSOD expectedly results in increased sensitivity to alcoholic liver injury primarily characterized by oxidant damage.68 Mice deficient in IL-6 are also susceptible to alcohol-induced apoptosis and steatosis of the liver,69 potentially resulting from insufficient STAT activation.79 To this end, it is intriguing that there appears to be cell-type dependent roles of STAT in alcoholic liver injury such that STAT3 in hepatocytes promotes inflammation, whereas STAT3 in macrophages and Kupffer cells suppresses inflammation,79 highlighting the importance of cell-type specific research for the genetic second hit manipulation. Leptin deficiency results in sensitization for alcoholic steatohepatitis, which was attributed to suppressed metallothionein expression.7 However, different aspects of metabolic complications associated with obesity and NAFLD, may also be involved in this heightened sensitivity. Whether naturally occurring obesity and IR synergistically enhance alcoholic liver injury, needs to be determined as the models such as fa/fa or ob/ob or that based on choline and methionine deficiency, are not relevant to the background commonly seen in obese patients or patients with diabetes. As discussed above, genetic manipulation of any gene involved in the transmethylation or transsulfuration pathways, will be of interest for the second hit analysis. To this end, a preliminary study on cystathionine β synthase (CBS) heterozygosity demonstrates enhanced alcoholic liver pathology and a marked reduction in SAMe/SAH ratio.70 The latter effect should severely impair methyltransferase activities and DNA methylation.

FUTURE DIRECTIONS FOR IDEAL ALD ANIMAL MODELS

Despite many decades of rigorous attempts, the field still suffers from a lack of animal models that reproduce the whole spectrum of ALD. However, this in facts tells us the essence of the ALD pathogenesis – the complexity of gene–environment interactions that underlie the pathogenesis. One environmental factor that we know is critical is excessive intake of alcohol. Even though a threshold dose may differ depending on the existence of genetic and environmental risk factors, the fact is that patients consume at least 40 g/day for a minimum of 10 years. This has to be incorporated as a basic requirement when testing “second hit” in animal models if maximal recapitulation of human ALD pathology is desired.

The second requirement is that “second hit” has to be natural and best reflect patient conditions. Knocking out one gene or excessive pharmacologic treatments may not be natural, but hemizygosity or reproducing natural lifestyle environments may be more conducive to development of better two hit models. These more “subtle” or natural hits should be combined with “excessive” alcohol intake to test “multiple hits” in the genesis of ALD.

Lastly, we need to pay more attention to comorbidities. Patients with severe end-stage ALD who require liver transplantation, often have comorbidities such as viral hepatitis, obesity, and diabetes. Animal models reproducing synergistic pathological outcome caused by these comorbidities, are urgently needed for mechanistic research and identification of therapeutic targets.

ACKNOWLEDGMENTS

Preparation of this manuscript was supported by the National Institute on Alcohol Abuse and Alcoholism grants P50AA11999, R21AA016682, R24AA12885, and VA Merit Review and Senior Career Scientist awards. The authors also thank the excellent administrative support by Rosy Macias and Rebecca Handan.

ABBREVIATIONS

ALD

alcoholic liver disease

ALT

alanine aminotransferase

ASH

alcoholic steatohepatitis

BHMT

betaine-homocysteine methyltransferase

CBS

cystathionine β synthase

CuZnSOD

copper zinc superoxide dismutase

CYP2E1

cytochrome P450 2E1

ER

endoplasmic reticulum

GNMT

glycine N-methyltransferase

HCC

hepatocellular carcinoma

HCV

hepatitis C virus

HFD

high fat diet

IKK

IkappaB kinase

IL

interleukin

IR

insulin resistance

IRS

insulin receptor substrate

Jak

Janus kinases

JNK

c-Jun N-terminal kinase

LPS

lipopolysaccharide

MAA

malondialdehyde-acetaldehyde adducts

mRNA

messenger ribonucleic acid

NAFLD

nonalcoholic fatty liver disease

NASH

nonalcoholic steatohepatitis

NFkB

nuclear factor kappa B

PAI-1

plasminogen activator inhibitor-1

PERK

PKR-like ER kinase

PPAR

peroxisome proliferator-activated receptor

SAH

S-adenosylhomocysteine

SAMe

S-adenosylmethionine

SOCS

suppressor of cytokine signaling

SREBP-1c

sterol regulatory element binding protein-1c

STAT

signal transducer and activator of transcription

TLR

toll-like receptor

TNFα

tumor necrosis factor-α;

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