Table 1.
Plasmids used
| Name | Description | Reference |
|---|---|---|
| B176 | YCp50: CEN4, URA3 cloning vector | Rose et al. 1987 |
| B181 | YCpLac33: CEN4, URA3 cloning vector | Gietz and Sugino 1988 |
| B184 | YEpLac 195: URA3 high copy vector | Gietz and Sugino 1988 |
| B187 | YIpLac211: URA3 integrative vector | Gietz and Sugino 1988 |
| B234 | 3.9 kb XhoI/XmaI PCR fragment of RRP44/DIS3 in SalI/XmaI-digested YCpLac33 | This study |
| B251 | 3.9 kb XhoI/XmaI product of RRP44/DIS3 in SalI/XmaI YEpLac195 | This study |
| B254 | 3.0 kb SspI/PmeI fragment pSup1 containing TRF4 into SmaI-digested YCpLac33 | This study |
| B256 | 3.1 kb SacI/PstI fragment of TRF4 from B254 into SacI/PstI YEpLac195 | This study |
| B269 | 3.4 kb XbaI/EcoRI fragment of RRP6 in XbaI/EcoRI YCpLac33 | Briggs et al. 1998 |
| CB432 | 3.8 kb SmaI/HindIII genomic fragment containing TRF4 into SmaI/HindIII pRS306 | Sadoff et al. 1995a |
| B227 | YCp50 containing chromosome XV DNA coordinates 280049 to 286635 | This study |
| B228 | YCp50 containing chromosome XV DNA coordinates 280689 to 287099 | This study |
| B229 | YCp50 containing chromosome XV DNA coordinates 276767 to 287099 | This study |
| B285 | 1.1 kb EcoRI fragment of IFM1 cloned into EcoRI-digested YIpLac211 | This study |
| B145 | A 3.0 kb XbaI/XhoI fragment containing TRM6 into SalI/XbaI-digested YIpLac211 | This study |
| a pSup 1.1 | YCp50 containing chromosome XV DNA coordinates 100202-112210 | This study |
| AKYOL114C | 2.5 kb YOL114C HindIII fragment from CB432 into HindIII-digested YCpLac33 | This study |
| p2704 | 3.0 kb XbaI/XhoI fragment containing TRM6 into XbaI/SalI-digested YCplac33 | Anderson et al. 1998 |
a
Genomic DNA contained in pSup1.1 and pSup1.2 is identical.