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. 1972 Mar;69(3):571–575. doi: 10.1073/pnas.69.3.571

Expression of Differentiated Functions in Hepatoma Cell Hybrids: Induction of Mouse Albumin Production in Rat Hepatoma-Mouse Fibroblast Hybrids*

Jerry A Peterson 1, Mary C Weiss 1
PMCID: PMC426509  PMID: 4335066

Abstract

The synthesis of serum albumin has been studied in hybrids between well-differentiated rat hepatoma cells, which synthesize serum albumin, and mouse fibroblasts (3T3) that do not synthesize albumin. By immunodiffusion techniques with noncrossreacting antisera, the production of both rat and mouse albumin by the hybrids has been examined. Karyologically identified hybrids were produced between 3T3 cells and cells of a 1s hepatoma (Fu5) clone, and of a 2s hepatoma (2s Fu5-5cl.lE) clone. Each of the 3T3 x Fu5 hybrids produces only rat albumin. Among five 3T3 x 2s Fu5-5cl.lE hybrid clones isolated, one produces both rat and mouse albumin, two produce only mouse albumin, and two do not produce rat or mouse albumin.

Keywords: immunodiffusion

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABDEL-SAMIE Y., BRODA E., KELLNER G., ZISCHKA W. Production of serum albumin and of globulins by chick mesenchymal tissue and by HeLa tumour tissue in culture. Nature. 1959 Aug 1;184(Suppl 6):361–362. doi: 10.1038/184361a0. [DOI] [PubMed] [Google Scholar]
  2. Avrameas S., Ternynck T. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry. 1969 Jan;6(1):53–66. doi: 10.1016/0019-2791(69)90178-5. [DOI] [PubMed] [Google Scholar]
  3. BOWER B. F., GORDAN G. S. HORMONAL EFFECTS OF NONENDOCRINE TUMORS. Annu Rev Med. 1965;16:83–118. doi: 10.1146/annurev.me.16.020165.000503. [DOI] [PubMed] [Google Scholar]
  4. Coon H. G., Weiss M. C. A quantitative comparison of formation of spontaneous and virus-produced viable hybrids. Proc Natl Acad Sci U S A. 1969 Mar;62(3):852–859. doi: 10.1073/pnas.62.3.852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Davidson R. L., Benda P. Regulation of specific functions of glial cells in somatic hybrids. II. Control of inducibility of glycerol-3-phosphate dehydrogenase. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1870–1877. doi: 10.1073/pnas.67.4.1870. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davidson R. L., Ephrussi B., Yamamoto K. Regulation of pigment synthesis in mammalian cells, as studied by somatic hybridization. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1437–1440. doi: 10.1073/pnas.56.5.1437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fougère C., Ruiz F., Ephrussi B. Gene dosage dependence of pigment synthesis in melanoma x fibroblast hybrids (hamster cells-mouse fibroblast-DOPA-oxidase-irradiation). Proc Natl Acad Sci U S A. 1972 Feb;69(2):330–334. doi: 10.1073/pnas.69.2.330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Green H., Ephrussi B., Yoshida M., Hamerman D. Synthesis of collagen and hyaluronic acid by fibroblast hybrids. Proc Natl Acad Sci U S A. 1966 Jan;55(1):41–44. doi: 10.1073/pnas.55.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Green H., Goldberg B., Todaro G. J. Differentiated cell types and the regulation of collagen synthesis. Nature. 1966 Nov 5;212(5062):631–633. doi: 10.1038/212631b0. [DOI] [PubMed] [Google Scholar]
  10. Gurdon J. B., Uehlinger V. "Fertile" intestine nuclei. Nature. 1966 Jun 18;210(5042):1240–1241. doi: 10.1038/2101240a0. [DOI] [PubMed] [Google Scholar]
  11. HAM R. G. CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM. Proc Natl Acad Sci U S A. 1965 Feb;53:288–293. doi: 10.1073/pnas.53.2.288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Halpern M., Rubin H. Proteins released from chick embryo fibroblasts in culture. I. Partial characterization of the proteins. Exp Cell Res. 1970 Apr;60(1):86–95. doi: 10.1016/0014-4827(70)90491-x. [DOI] [PubMed] [Google Scholar]
  13. Kaighn M. E., Prince A. M. Production of albumin and other serum proteins by clonal cultures of normal human liver. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2396–2400. doi: 10.1073/pnas.68.10.2396. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Klebe R. J., Chen T., Ruddle R. H. Mapping of a human genetic regulator element by somatic cell genetic analysis. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1220–1227. doi: 10.1073/pnas.66.4.1220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
  16. Minna J., Nelson P., Peacock J., Glazer D., Nirenberg M. Genes for neuronal properties expressed in neuroblastoma x L cell hybrids. Proc Natl Acad Sci U S A. 1971 Jan;68(1):234–239. doi: 10.1073/pnas.68.1.234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mohit B., Fan K. Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma. Science. 1971 Jan 8;171(3966):75–77. doi: 10.1126/science.171.3966.75. [DOI] [PubMed] [Google Scholar]
  18. Ohanian S. H., Taubman S. B., Thorbecke G. J. Rates of albumin and transferrin synthesis in vitro in rat hepatoma-derived H4II-EC cells. J Natl Cancer Inst. 1969 Aug;43(2):397–406. [PubMed] [Google Scholar]
  19. Peters T., Jr Serum albumin. Adv Clin Chem. 1970;13:37–111. doi: 10.1016/s0065-2423(08)60385-6. [DOI] [PubMed] [Google Scholar]
  20. Schneider J. A., Weiss M. C. Expression of differentiated functions in hepatoma cell hybrids. I. Tyrosine aminotransferase in hepatoma-fibroblast hybrids. Proc Natl Acad Sci U S A. 1971 Jan;68(1):127–131. doi: 10.1073/pnas.68.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Steward F. C. From cultured cells to whole plants: the induction and control of their growth and morphogenesis. Proc R Soc Lond B Biol Sci. 1970 Feb 17;175(1038):1–30. doi: 10.1098/rspb.1970.0009. [DOI] [PubMed] [Google Scholar]
  22. TODARO G. J., GREEN H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol. 1963 May;17:299–313. doi: 10.1083/jcb.17.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tjio J. H., Puck T. T. THE SOMATIC CHOMOSOMES OF MAN. Proc Natl Acad Sci U S A. 1958 Dec 15;44(12):1229–1237. doi: 10.1073/pnas.44.12.1229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Uriel J. Méthode d'électrophorèse dans des gels d'acrylamide-agarose. Bull Soc Chim Biol (Paris) 1966;48(8):969–982. [PubMed] [Google Scholar]

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