Table 3.
Summary of Signal Calculations and Plate Acceptance Criteria
| Requirements | NAD+NADH | NADP+NADPH |
|---|---|---|
| Intraplate tests | ||
| All HI signal CVs <20% | 8.5–10.7 | 5.0–7.5 |
| All MID signal (unnormalized) CVs <20 | 8.9–10.7 | 4.9–9.3 |
| All normalized MID (Mid%) SDs <20 | 9.1–12 | 6.5–12.5 |
| All LO CVs <20% | 6.8–9.2 | 6.3–9.3 |
| All SW's >2 | 6.6–8.4 | 7.2–13.7 |
| All Z factors >0.4 | 0.7–0.8 | 0.7–0.8 |
| Interplate tests | ||
| All within-day fold shifts <2 | 1.0–1.6 | 1.0–1.9 |
| All Average (between-) day fold shifts <2 | 1.2 | 1.5 |
HI, the maximum signal generated with untreated cells; MID, the mid-level signal generated with cells treated with FK866 inhibitor at the concentration corresponding to IC50 values; LO, the minimum signal generated with cells treated at the high FK866 concentration corresponding to >90% inhibition; SW, assay signal window.