Figure 3.
Effect of Indole on NAD(P)H and ATP/ADP Ratio
(A and B) Representative traces of NAD(P)H autofluorescence (A) and the ATP/ADP ratio monitored by Perceval fluorescence (B), in three individual GLUTag cells. The 1 mM indole was added to the perfusion solution, as indicated by the red bars, and 1 μM rotenone was perfused as indicated by the blue bars.
(C) Mean rates of change in the signals for NAD(P)H and ATP/ADP ratio calculated during addition of either 1 mM indole or 1 μM rotenone. The rate measured during the control (in the presence of saline plus 1 mM glucose) is set to zero by subtracting it from the rates measured at 1 mM indole, washout and 1 μM rotenone for each individual cell. In the graph the rates are the means ± SEM for 27 cells.