Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1977 Dec;74(12):5318–5322. doi: 10.1073/pnas.74.12.5318

Biosynthesis of the covalently linked diglyceride in murein lipoprotein of Escherichia coli.

P K Chattopadhyay, H C Wu
PMCID: PMC431701  PMID: 341153

Abstract

Biosynthesis of the diglyceride moiety of murein lipoprotein in Escherichia coli was studied by pulse-labeling with [2-3H]glycerol and subsequent chase. The evidence strongly suggests that the precursor of the glycerol moiety in lipoprotein is one of the major phospholipid species in E. coli. Studies of biosynthesis of lipoprotein in cerulenin-treated cells indicated that the nonacylated glycerol moiety of phosphatidylglycerol is the donor for the formation of a thioether linkage in the glycerylcysteine residue of the lipoprotein. This is supported by the observation that carbon 1 rather than carbon 3 of sn-glycerol is involved in this thioether linkage. We propose that the biosynthesis of lipoprotein proceeds as follows: apolipoprotein + phosphatidylglycerol or acyl phosphatidylglycerol leads to lipoprotein + phosphatidic acid.

Full text

PDF
5318

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braun V., Bosch V. Sequence of the murein-lipoprotein and the attachment site of the lipid. Eur J Biochem. 1972 Jun 23;28(1):51–69. doi: 10.1111/j.1432-1033.1972.tb01883.x. [DOI] [PubMed] [Google Scholar]
  2. Braun V., Rehn K. Chemical characterization, spatial distribution and function of a lipoprotein (murein-lipoprotein) of the E. coli cell wall. The specific effect of trypsin on the membrane structure. Eur J Biochem. 1969 Oct;10(3):426–438. doi: 10.1111/j.1432-1033.1969.tb00707.x. [DOI] [PubMed] [Google Scholar]
  3. Cho K. S., Hong S. D., Cho J. M., Chang C. S., Lee K. S. Studies on the biosynthesis of acylphosphatidylglycerol in Escherichia coli B and B/r. Biochim Biophys Acta. 1976 Jan 18;486(1):47–54. doi: 10.1016/0005-2760(77)90068-6. [DOI] [PubMed] [Google Scholar]
  4. Dankert M., Wright A., Kelley W. S., Robbins P. W. Isolation, purification, and properties of the lipid-linked intermediates of O-antigen biosynthesis. Arch Biochem Biophys. 1966 Sep 26;116(1):425–435. doi: 10.1016/0003-9861(66)90049-x. [DOI] [PubMed] [Google Scholar]
  5. Halegoua S., Sekizawa J., Inouye M. A new form of structural lipoprotein of outer membrane of Escherichia coli. J Biol Chem. 1977 Apr 10;252(7):2324–2330. [PubMed] [Google Scholar]
  6. Hantke K., Braun V. Covalent binding of lipid to protein. Diglyceride and amide-linked fatty acid at the N-terminal end of the murein-lipoprotein of the Escherichia coli outer membrane. Eur J Biochem. 1973 Apr;34(2):284–296. doi: 10.1111/j.1432-1033.1973.tb02757.x. [DOI] [PubMed] [Google Scholar]
  7. Harder M. E., Ladenson R. C., Schimmel S. D., Silbert D. F. Mutants of Escherichia coli with temperature-sensitive malonyl coenzyme A-acyl carrier protein transacylase. J Biol Chem. 1974 Dec 10;249(23):7468–7475. [PubMed] [Google Scholar]
  8. Inouye S., Lee N., Inouye M., Wu H. C., Suzuki H., Nishimura Y., Iketani H., Hirota Y. Amino acid replacement in a mutant lipoprotein of the Escherichia coli outer membrane. J Bacteriol. 1977 Oct;132(1):308–313. doi: 10.1128/jb.132.1.308-313.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Inouye S., Wang S., Sekizawa J., Halegoua S., Inouye M. Amino acid sequence for the peptide extension on the prolipoprotein of the Escherichia coli outer membrane. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1004–1008. doi: 10.1073/pnas.74.3.1004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kennedy E. P., Rumley M. K., Schulman H., Van Golde L. M. Identification of sn-glycero-1-phosphate and phosphoethanolamine residues linked to the membrane-derived Oligosaccharides of Escherichia coli. J Biol Chem. 1976 Jul 25;251(14):4208–4213. [PubMed] [Google Scholar]
  11. Lin J. J., Wu H. C. Biosynthesis and assembly of envelope lipoprotein in a glycerol-requiring mutant of Salmonella typhimurium. J Bacteriol. 1976 Mar;125(3):892–904. doi: 10.1128/jb.125.3.892-904.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Olsen R. W., Ballou C. E. Acyl phosphatidylglycerol. A new phospholipid from Salmonella typhimurium. J Biol Chem. 1971 May 25;246(10):3305–3313. [PubMed] [Google Scholar]
  13. Omura S. The antibiotic cerulenin, a novel tool for biochemistry as an inhibitor of fatty acid synthesis. Bacteriol Rev. 1976 Sep;40(3):681–697. doi: 10.1128/br.40.3.681-697.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Raetz C. R. Phosphatidylserine synthetase mutants of Escherichia coli. Genetic mapping and membrane phospholipid composition. J Biol Chem. 1976 Jun 10;251(11):3242–3249. [PubMed] [Google Scholar]
  15. Schulman H., Kennedy E. P. Relation of turnover of membrane phospholipids to synthesis of membrane-derived oligosaccharides of Escherichia coli. J Biol Chem. 1977 Jun 25;252(12):4250–4255. [PubMed] [Google Scholar]
  16. Swank R. T., Munkres K. D. Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate. Anal Biochem. 1971 Feb;39(2):462–477. doi: 10.1016/0003-2697(71)90436-2. [DOI] [PubMed] [Google Scholar]
  17. Venkateswaran P. S., Wu H. C. Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12. J Bacteriol. 1972 Jun;110(3):935–944. doi: 10.1128/jb.110.3.935-944.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wu H. C., Lin J. J. Escherichia coli mutants altered in murein lipoprotein. J Bacteriol. 1976 Apr;126(1):147–156. doi: 10.1128/jb.126.1.147-156.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES