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. 2015 Feb 13;10(2):e0117266. doi: 10.1371/journal.pone.0117266

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© 2015 Rotschafer et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — The fractional coverage of VGLUT and VGAT was assessed in the VCN (A), MNTB (B), and LSO (C). No significant differences were found in VGLUT or VGAT fractional coverage in VCN (A). Fmr1 ^-/- (gray) MNTB had significantly more VGAT coverage than wild type MNTB (black), though there was no difference in VGLUT coverage. VGLUT and VGAT fractional coverage was significantly greater in Fmr1 ^-/- within the LSO. VGLUT and VGAT fractional coverage was compared by calculating a synaptic protein index value for each nucleus (D). No significant differences were found in VCN or LSO, but Fmr1 ^-/- MNTB had lower index values than wild type MNTB. The smaller I_SP value likely reflects the heightened VGAT staining in Fmr1 ^-/- MNTB.