FIG. 2.
Effects of time course, dose of virus, and virus strain on PLA2 activity of HCMV. (A) Effect of time course on hydrolysis of BODIPY-PC by HCMV. HCMV (AD169, 106 PFU) was incubated with BODIPY-PC over a time course of 100 min. (B) Effect of increasing amounts of HCMV on hydrolysis of BODIPY-PC. (C) HCMV was incubated with BODIPY-PC for 30 min at 37°C. HCMV strains Toledo (5.105 PFU), TB40/E (2.105 PFU), AD169 (106 PFU), and Towne (5.105 PFU) were analyzed for hydrolysis of NBD-PC and BODIPY-PC. Background hydrolysis was substracted from experimental values. Lipid extraction and HPLC analysis of fluorescent metabolites were performed as described in Materials and Methods. Data are representative of three similar independent experiments.