Table 1.
Antibodies used for immunohistochemistry or western immunoblotting
Antibodya | Typeb | Assayc | Dilution | Company |
---|---|---|---|---|
20Sα5 | P | I | 1 : 1000 | Affinity BioReagents, Golden, CO, USA |
20Sα7 | M | W | 1 : 1000 | Biomol, Plymouth Meeting, PA, USA |
20Sβ1i (LMP2) | M | W | 1 : 1000 | Biomol |
20Sβ5i (LMP7) | P | I | 1 : 1000 | Biomol |
20Sβ5i (LMP7) | P | W | 1 : 1000 | Biomol |
20Sβ1 (20SY) | P | W | 1 : 1000 | Affinity BioReagents |
20Sβ5 (20SX) | P | W | 1 : 1000 | Affinity BioReagents |
20Sβ5 (20SX) | P | I | 1 : 200 | Affinity BioReagents |
Arrestin (A9C6) | M | I | 1 : 1000 | Dale Gregerson, University of Minnesota, USA |
GFAP | M | I | 1 : 2000 | Chemicon, Temecula, CA, USA |
GFAP | M | W | 1 : 1000 | Chemicon |
MBP | M | I | 1 : 500 | Chemicon |
CD45 | M | I | 1 : 20 | Biolegend, San Diego, CA, USA |
Glial fibrillary acidic protein (GFAP), myelin basic protein (MBP);
Monoclonal (M), polyclonal (P);
Immunohistochemistry or Immunofluorescence (I), Western blotting (W).