Fig E1.
Analysis of cell phenotype. A and B, Expanded TH2 cells were CD3+CD4+CRTH2+CD45RO+GATA3+CCR6−CCR7−CD45RA−RORγt− effector memory cells (Fig E1, A), which produce the type II cytokines IL-8 and IL-22 after stimulation with PMA (5 ng/mL) and ionomycin (500 ng/mL; Fig E1, B), as detected by means of flow cytometry. Blue lines represent staining of indicated cell markers, and red lines represent unstained controls. C, The same cytokine profile was observed in freshly isolated ex vivo TH2 cells by using qPCR (mRNA) and Luminex (protein) assays (high background IL-8 levels in the unstimulated sample precluded accurate analysis of this cytokine in the Luminex assay; n = 6 for Fig E1, A and B; n = 2 for mRNA and n = 1 for protein in Fig E1, C).