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. 2015 Apr 14;129(6):895–907. doi: 10.1007/s00401-015-1415-2

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© The Author(s) 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Immunoblotting and immunoelectron microscopic analyses of tau in cells expressing both APP and HA-tagged 4R1N tau. Immunoblot analysis of lysates from cells transfected with both HA-4R1N tau and APP, cells transfected with HA-4R1N tau and treated with 4R1N tau fibrils, and cells transfected with both HA-4R1N tau and APP and treated with 4R1N tau fibrils (a). Cells were sequentially extracted to obtain Tris-soluble (TS), Triton X-100-soluble (TX), and Sarkosyl-soluble (Sar) fractions, leaving the pellet fraction (ppt). HA-4R1N tau was detected with pS396 (p-Ser-396) and anti-HA antibodies. Immunoelectron microscopy of tau in the Sar-insoluble fraction from cells transfected with both HA-4R1N tau and APP and treated with 4R1N tau fibrils (b). Anti-HA-positive (left panel) and AT8 (p-Ser-202 and p-Thr-205)-positive (right panel) filaments were observed. Scale bars represent 100 nm