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. 2014 Dec 30;24(8):2228–2240. doi: 10.1093/hmg/ddu741

Figure 6.

Figure 6.

Low protein diets corrected autophagosome defects in G59S-mice. (A) Left: representative western blot of LC3-I (upper band) and LC3-II (lower band) in the cerebral cortex of wild type or G59S mice, fed with chow or low protein diet. Actin western blot is provided as a standard. Right: LC3 II/LC3 I ratio, as measured using western blotting in the cortex and spinal cord of wild-type (black columns) and G59S-mice (grey columns) fed with chow diet (−LPD) or low protein diet (+LPD). **P < 0.01; ****P < 0.0001 versus corresponding wild type. (B and C) electron micrographs of neuronal cells in spinal cord from age-matched wild-type (WT) and G59S-mice (G59S) under chow or LPD, and either injected with saline (NaCl) or chloroquine. Arrows indicate autophagosomes including degradative organelles (B) or protein inclusions (C); scale bar is 1000 nm. (D) quantitative analysis of autophagosomes (D), and protein inclusions (E) per spinal neuronal cells in G59S-mice and wild-type age-matched controls under the different treatment groups, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 versus corresponding wild type.