Figure 7. GBA1 deficiency increases the propagation of α-synuclein pathology in vivo.
(a) Immunohistochemistry of α-synuclein with three different antibodies. Boxed areas in the top panels are magnified in the panels below. Engrafted cells are indicated with arrowheads. Scale bars: top panel, 250 µm; lower panels, 20 µm. (b, c) α-synuclein immunoreactivities quantified and expressed as a percentage of the grafted area; n = 8, *p < 0.01 by t-test. (d, e) Co-immunofluorescence analysis of engrafted cells (arrowheads). Pixel intensities of α-synuclein labels measured in 40 cells per animal (n = 8). Scale bar: 5 µm. (f, g) Co-immunofluorescence analysis of engrafted cells stained for TH (arrowheads). Pixel intensities of α-synuclein labels measured in 40 cells per animal (n = 6). Scale bar: 5 µm.