| Cell Number | Antibody concentration | Bead Volume (bed volume) |
|---|---|---|
| 10,000 | 0.25 μg per 1 ml | 7–10 μl |
| 25,000 | 0.4 μg per 1 ml | 10 μl |
| 500,000 | 1 μg per 1 ml | 20 μl |
| 10,000,000 | 10 μg per 10 ml | 70 μl |
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