Figure 2. Stabilization of GCGR by peptide ligand in the HDX studies.
Changes to average percent deuterium are shown on the full-length GCGR model based on ECD and 7TM crystal structures. Dark blue regions of receptor indicate areas of large decreased exchange in the presence of the des-His1-[Nle9-Ala11-Ala16]-glucagon-NH2 peptide ligand (depicted as green ribbon) and cyan indicates regions with smaller decreased exchange, while black indicates no significant change and white indicates regions where no peptide ions were detected using mass spectrometry. HDX plots for selected regions are shown around the structure. The data are shown as mean±s.d. of three independent experiments. Average percent deuterium values and percent deuterium values at 10 s are reported in Supplementary Fig. 4 and Table 1, respectively.