Figure 2.
miR-146b and miR-146a are induced in endotoxin tolerized monocytes. Monocytes were first primed with 0, 0.1, or 10 ng/mL LPS (1°LPS) for 18 h and then challenged for 24 h with 10 ng/mL LPS (2°LPS). After 24 h stimulation, TNFα levels (a) were measured by ELISA in cell-free supernatants, and levels of (b) miR-146b and (c) miR-146a were assayed by qPCR in triplicate samples, normalized relative to U6, and expressed as fold enrichment as compared to unstimulated samples. Results are expressed as mean ± SEM of three independent experiments.