Figure 2.
Carfilzomib and ONX 0914 reduce numbers of plasma cells and germinal center cells in Lupus prone mice. (a) 10 week-old MRL/lpr mice were treated for 13 weeks with PIs as in Figure 1 and PC (CD138+, k light chain+) counts defined by flow cytometry in spleen (left) and bone marrow (right). *p=0.0003, **p<0.0001, ***p=0.01, ****p=0.005 (b) The numbers of total IgG ASC (top panels) and anti-dsDNA IgG ASC (bottom panels) from spleen and bone marrow were measured by ELISPOT assays. *p=0.008, **p=0.005, ***p=0.03, ****p=0.01, #p=0.05). (c) Representative spleen sections of normal mice, NZB/W mice treated with vehicle, and NZB/W mice treated with ONX 0914 for 8 weeks (2+protein at start). (d) GC cell (B220+, GL-7+) and PC numbers in spleen of NZB/W mice after 8 weeks of ONX 0914 (n = 3, 20 mg/kg) vs. vehicle solution (n = 3). Representative spleen sections stained with GL7. All data is expressed as mean + s.e.m..