Figure 6.
PIs suppress both the survival and function of IFN-α producing pDCs. (a) Flow cytometry analysis of mouse BM cells 1 hour after in vivo injection of PIs as described in the Figure 4c. The mean (n = 2) + s.e.m of %pDCs in total lymphocytes from BM of mice treated with different PIs is shown. (b) Representative flow cytometry analysis of pDCs after 16 hrs incubation in vitro as described in Figure 4 (a). Line graph depicts BM lymphocyte pDC % from in vitro culture incubated with different PIs at titrating concentrations (representative of 3 experiments). (c) C57B6 mouse BM cells were incubated with titrating amounts of the indicated PIs in vitro in the presence of CpG2216 (500 ng/ml) for 5 hours and an additional 3 hours with GolgiPlug. Intracellularly stained IFN-α accumulating pDC and total pDC levels were normalized to the vehicle treated control and plotted against the PI concentrations. Data presented are representative of 3 experiments, and the curves were fitted to the non-linear regression single decay.