Fig. 4.
Subcellular distribution of bile acid fluorescent analogs in hepatocytes. Representative confocal microscopy analysis of the subcellular distribution of DCA-NBD (A) and UDCA-NBD (D) in hepatocytes after internalization. Rat liver hepatocytes were incubated with 20 μM of bile acid analogs during 30 min at 37°C. Mitochondria were fluorescently stained with 5 μM Rho-123 for 15 min at 37°C (B, E). Overlay images are shown (C, F). Inset images show the presence of bile acid analogs in the outer mitochondrial membrane.