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. 2016 Jan 1;143(1):54–65. doi: 10.1242/dev.130005

Fig. 5.

Fig. 5.

Aqp5Cre-driven ectopic SOX2 expression reprograms flattened AT1 cells. (A) Confocal projection images of immunostained Aqp5Cre/+; RosaSox2/+ lung strips at the indicated stages. Some mutant AT1 cells (arrowhead) have retracted their cellular extensions and accumulate a high level of E-CAD at P3. Mutant cell clusters (arrow) are present at P8. (B) Confocal images of sections from littermate control and mutant lungs showing comparable phenotypes of AT1 cells targeted with Aqp5Cre to those targeted with Scnn1a-Cre (Fig. 4). Mutant AT1 cells (GFP, arrowhead) form clusters, downregulate RAGE (boxed region shown as single-channel images in insets), express KI67 and DNp63 (deltaNp63), and have diffuse (arrow) or nuclear (solid arrowhead) NKX2.1 (single-channel image shown in inset). NKX2.1 expression in AT2 cells (open arrowhead, cuboidal E-CAD staining) of the mutant lung is not affected. KI67 is expressed in non-AT1 cells in the control lung (Fig. S3A). Scale bars: 10 μm.