Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1970 Mar;23(2):172–177. doi: 10.1136/jcp.23.2.172

A simple diagnostic milk medium for Pseudomonas aeruginosa

M R W Brown 1,1, J H Scott Foster 1
PMCID: PMC474488  PMID: 4987392

Abstract

An agar medium containing 10% defatted milk has been tested as a diagnostic medium for Pseudomonas aeruginosa and, in particular, for differentiating between P. aeruginosa and P. fluorescens. Only P. aeruginosa colonies gave clear zones due to hydrolysis of casein together with diffused green pigment. Results on milk agar correlated well with the pattern of results from a variety of conventional tests used to identify this organism. Pigment production of P. aeruginosa on milk agar was better than on special media commonly used to enhance this characteristic. Routine diagnosis of P. aeruginosa is recommended by streaking on a solid medium containing 10% defatted milk granules, 25% nutrient broth, and 2% agar, and examining for clear zones and pigment after 24 hours' incubation.

Full text

PDF
172

Images in this article

175Image
on p.175

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COLWELL R. R. A STUDY OF FEATURES USED IN THE DIAGNOSIS OF PSEUDOMONAS AERUGINOSA. J Gen Microbiol. 1964 Nov;37:181–194. doi: 10.1099/00221287-37-2-181. [DOI] [PubMed] [Google Scholar]
  2. GABY W. L., FREE E. Occurrence and identification of nonpigmented strains of Pseudomonas aeruginosa in the clinical laboratory. J Bacteriol. 1953 Jun;65(6):746–746. doi: 10.1128/jb.65.6.746-746.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HAYNES W. C. Pseudomonas aeruginosa--its characterization and identification. J Gen Microbiol. 1951 Nov;5(5 Suppl):939–950. doi: 10.1099/00221287-5-5-939. [DOI] [PubMed] [Google Scholar]
  4. HUGH R., LEIFSON E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol. 1953 Jul;66(1):24–26. doi: 10.1128/jb.66.1.24-26.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KING E. O., WARD M. K., RANEY D. E. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954 Aug;44(2):301–307. [PubMed] [Google Scholar]
  6. KOVACS N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature. 1956 Sep 29;178(4535):703–703. doi: 10.1038/178703a0. [DOI] [PubMed] [Google Scholar]
  7. Khan N. C., Sen S. P. Genetic transformation in Pseudomonas. J Gen Microbiol. 1967 Nov;49(2):201–209. doi: 10.1099/00221287-49-2-201. [DOI] [PubMed] [Google Scholar]
  8. LYSENKO O. Pseudomonas--an attempt at a general classification. J Gen Microbiol. 1961 Jul;25:379–408. doi: 10.1099/00221287-25-3-379. [DOI] [PubMed] [Google Scholar]
  9. PRESTON N. W., MORRELL A. Reproducible results with the Gram stain. J Pathol Bacteriol. 1962 Jul;84:241–243. doi: 10.1002/path.1700840131. [DOI] [PubMed] [Google Scholar]
  10. Phillips I. Identification of Pseudomonas aeruginosa in the clinical laboratory. J Med Microbiol. 1969 Feb;2(1):9–16. doi: 10.1099/00222615-2-1-9. [DOI] [PubMed] [Google Scholar]
  11. RHODES M. E. The characterization of Pseudomonas fluorescens with the aid of an electronic computer. J Gen Microbiol. 1961 Jul;25:331–345. doi: 10.1099/00221287-25-3-331. [DOI] [PubMed] [Google Scholar]
  12. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
  13. WAHBA A. H., DARRELL J. H. THE IDENTIFICATION OF ATYPICAL STRAINS OF PSEUDOMONAS AERUGINOSA. J Gen Microbiol. 1965 Mar;38:329–342. doi: 10.1099/00221287-38-3-329. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES