Figure 2. InsG3680+/+ but Not R1117X+/+ Mice Display Altered Striatal Synaptic Transmission at P14 and Reduced Social Interaction at P23.
(A and D) Representative cortico-striatal pop spike traces recorded from P14 mice with indicated genotypes.
(B and C) Cortical-striatal input-output curve shows reduced pop spike responses in InsG3680+/+ mice compared to wild-type littermates. NP1 amplitude is similar between the genotypes, suggesting that presynaptic input is not different.
(E and F) Cortical-striatal input-output curve shows similar pop spike responses and NP1 amplitude between R1117X+/+ mice and their wild-type littermates. In (B), (C), (E), and (F), data are presented as mean ± SEM. *p < 0.05; two-way ANOVA repeated-measures with Bonferroni post hoc test. n = 12 slices from 4 pairs of littermates for each cohort.
(G and H) Typical AMPA receptor-mediated mEPSC traces recorded from P14 MSNs with indicated genotypes.
(I and J) Amplitude but not frequency of mEPSC is increased in InsG3680+/+ MSNs compared to wild-type. No change of amplitude or frequency of mEPSC in R1117X+/+ MSNs. In InsG3680 cohort, n = 29 neurons for wild-type; n = 29 neurons for InsG3680+/+ from 3 pairs of littermates; in R1117X cohort, n = 30 neurons for wild-type, n = 31 neurons for R1117X+/+ from 3 pairs of littermates. Data are presented as mean ± SEM; two-tailed t test.
(K and M) Total number of interaction events from mice pairs with indicated genotypes as normalized to wild-type control.
(L and N) Normalized number of categorized interaction events from mice pairs with indicated genotypes as normalized to wild-type control.
In (K), (L), (M), and (N), in R1117X cohort, n = 9 pairs of mice for wild-type; n = 9 pairs of mice for R1117X+/+; in InsG3680 cohort, n = 8 pairs of mice for wild-type; n = 10 pairs of mice for InsG3680+/+. Data are presented as mean ± SEM, *p < 0.05, **p < 0.01; two-tailed t test.