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Km and Vmax values of AML-associated Tet2 mutants for iron and 2-oxoglutarate
The values are mean ± S.D. of 3 to 7 independent assays.
| Variable measured | Unit | WT Tet2 | H1302Y | D1304A | H1802R | R1817M | R1817S |
|---|---|---|---|---|---|---|---|
| Km of Fe2+ | μm | 3.6 ± 3 | 110 ± 4 | 165 ± 90 | 200 ± 185 | NDa | ND |
| Km of 2-oxoglutarate | μm | 60 ± 15 | ND | ND | ND | >5000 | >5000 |
| Vmax | % of WT Tet2 | 100 | 45 | 55 | 50 | ND | ND |
a ND, not determined.
