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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Nat Methods. 2011 Apr 28;8(5):393–399. doi: 10.1038/nmeth.1596

Table 1.

Summary of the 2PA properties of representative fluorescent proteins. Two-photon absorption cross section (σ2), two-photon brightness (σ2’), both per mature protein are presented in two spectral regions, corresponding to the short- and long-wavelength transitions.

Short-wavelength band Long-wavelength band
Protein λ2PA (nm) σ2 (GM) σ2 × φ (GM) λ2PA (nm) σ2 (GM) σ2 × φ (GM)
EBFP2.041 552 16 11 750 13 9.2
ECFP (Clontech) 550 15 7.8 857 23 12
Cerulean41 550 27 16 858 23 13
mTFP1.042 667 6.4 5.4 875 70 59
EGFP (Clontech) 660 22 17 927 39 30
mAmetrine43 655 19 13 809 56 40
Citrine44 590 34 23 968 10 6.7
mKok45 686 93 67 1044 41 30
mOrange31 640 200 140 1080 67 47
TagRFP46 759 300 130 1050 95 42
tdTomato31 684 316 228 1050 278 200
DsRed247 700 112 79 1050 103 73
mCherry31 740 101 24 1080 27 6.4
mRaspberry48 704 346 65 1118 31 5.8
mPlum48 724 114 15 1105 22 2.9
tdKatushka249 710 645 285 1100 143 63
mKate249 712 216 91 1140 72 30
mNeptune50 750 335 57 1105 70 12

Note: To obtain σ2 and σ2’ per chromophore in the case of dimers, one has to take one-half of the corresponding values presented in the Table.