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. 2016 Mar 4;6:22276. doi: 10.1038/srep22276

Table 1. Glutathione related antioxidants.

  GSH (nmol/mg pro)
in vivo
 in vitro
CD DHA diet control DHA
RA/PBS 17.9 ± 0.5 22.1 ± 0.3*# 17.9 ± 0.5* 43.9 ± 0.9*#
RA/LPS 27.8 ± 1.3* 28.1 ± 0.6* 38.3 ± 0.4* 31.6 ± 1.4*
O2/PBS 33.4 ± 0.7* 26.8 ± 0.2*# 32.8 ± 0.6* 36.5 ± 0.5*
O2/LPS(6h) 32.2 ± 0.06* 31.6 ± 0.6* 32.2 ± 0.6* 27.9 ± 0.6*#
O2/LPS(24h) 26.0 ± 0.6* 32.1 ± 0.1*# 28.2 ± 0.4* 27.2 ± 0.1*
  effect of DHA, LPS, O2interactions between DHA*LPS, DHA O2, LPS*O2,DHA*LPS*O2
 effect of DHA, LPS, O2interactions between DHA*LPS, DHA O2, LPS*O2,DHA*LPS*O2
  GSSG (nmol/mg pro)
RA/PBS 6.0 ± 0.7 8.0 ± 0.4 6.0 ± 0.7 18.4 ± 0.8*#
RA/LPS 8.9 ± 0.7* 10.2 ± 0.4* 17.0 ± 0.1* 13.4 ± 0.8*
O2/PBS 11.1 ± 0.9* 9.3 ± 0.5* 11.1 ± 0.9 10.1 ± 2.8
O2/LPS(6h) 13.3 ± 0.3* 10.5 ± 0.4*# 13.2 ± 0.3* 12.3 ± 0.2*
O2/LPS(24h) 9.8 ± 0.5* 11.3 ± 0.1* 9.5 ± 0.5 10.1 ± 0.1
  effect of LPS, O2 effect of LPS, O2
  GR (μmol/min/mg pro)
RA/PBS 4.3 ± 0.2 21.3 ± 1.7*# 4.3 ± 0.2 46.5 ± 2.7*#
RA/LPS 1.7 ± 0.7 35.5 ± 1.4*# 46.0 ± 7.1* 25.7 ± 1.5*
O2/PBS 32.1 ± 0.9* 34.8 ± 0.1* 32.1 ± 0.9* 37.4 ± 2.1*
O2/LPS(6h) 41.2 ± 4.5* 33.2 ± 0.4* 12.7 ± 0.7 14.3 ± 1.6
O2/LPS(24h) 31.1 ± 5.9* 45.6 ± 1.8*# 22.8 ± 2.1* 23.2 ± 0.7*
  effect of LPS, O2 effect of LPS, O2
  GPx (μmol/min/mg pro)
RA/PBS 69.3 ± 19.0 69.3 ± 19.0 62.2 ± 18.2 34.9 ± 14.1
RA/LPS 97.7 ± 29.4 110.5 ± 29.6 82.2 ± 21.0 98.1 ± 24.7
O2/PBS 44.3 ± 20.8 44.3 ± 20.9 83.7 ± 21.1 95.0 ± 28.4
O2/LPS(6h) 86.8 ± 23.7 78.5 ± 19.9 51.4 ± 13.1 70.4 ± 17.7
O2/LPS(24h) 61.8 ± 21.1 122.5 ± 46.0 74.8 ± 19.1 103.4 ± 26.3
  no differences no differences

Members of the glutathione antioxidant system were measured in isolated macrophages as described in Methods. CD: isolated from mice fed control diet, in vivo; DHA diet: isolated from mice fed a DHA supplemented diet, in vivo; control: isolated from mice fed standard diet and treated with vehicle, in vitro; DHA: isolated from mice fed standard diet and treated with DHA, in vitro. The data reflect n = 3 from three independent experiments. Data were analyzed by Multivariate Linear Regression with Tukey’s post hoc. * indicates different that CD RA/PBS, # indicates different than same treatment (difference between diets), p < 0.05.