FIG. 7.
Keratinocytes lacking RelA and c-rel fail to form colonies in culture. Equivalent numbers of viable keratinocytes (106) were grown for 7 days. (A) Control (rela+/+ c-rel−/− tnfα−/−) and mutant (rela−/− c-rel−/− tnfα−/−) keratinocyte cultures were stained with anti-keratin-14 (+) or isotype-matched negative-control (−) antibodies. (B) Keratinocytes of differing genotypes (rela+/+ c-rel+/+ tnfα−/−, rela+/+ c-rel−/− tnfα−/−, rela−/− c-rel+/+ tnfα−/−, and rela−/− c-rel−/− tnfα−/−) are shown by phase contrast.