FIG. 4.
Effect of TCPOBOP on liver composition of individual bile acids in male and female mice. Corn oil vehicle or TCPOBOP (3 mg/kg) was administered daily (IP) for 4 consecutive days to male and female mice (n = 5–6 per treatment group). At 96 h after the first dose, livers were collected and individual BAs were quantified by UPLC-MS/MS. Each section in pie charts was calculated to represent the mean proportion of an individual BA relative to the total BA concentration. Asterisks (*) denote statistically different from vehicle control (P < 0.05). Primary bile acids (1° BAs), secondary bile acids (2° BAs), 6-hydroxylated (6-OH), 12α-hydroxylated (12α-OH), bile acid (BA), cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), females (F), lithocholic acid (LCA), males (M), muricholic acid (MCA), total bile acids (Σ-BAs), taurine-conjugated bile acids (T-BAs), 1,4-bis(2-[3,5-dichloropyridyloxy])benzene (TCPOBOP), unconjugated bile acids (U-BAs), ursodeoxycholic acid (UDCA), vehicle (veh). Color image is available in the online version of the article.