Table 2. Endogenous FAA levels in tissues from FAAH+/-, FAAH-/-, and FAAH-NS mice (pmol/g wet tissue).
| Brain | Spinal cord | Testis | Liver | Kidney | |
|---|---|---|---|---|---|
| FAAH+/- | |||||
| Anandamide | 10 ± 2 | 5.6 ± 1.3 | 11 ± 4 | 2.2 ± 0.1 | ND |
| PEA | 241 ± 63 | 1036 ± 45 | 149 ± 33 | 104 ± 23 | 221 ± 28 |
| OEA | 102 ± 17 | 411 ± 36 | 76 ± 10 | 43 ± 5 | 116 ± 10 |
| FAAH-/- | |||||
| Anandamide | 62 ± 6** | 173 ± 9** | 197 ± 28†† | 12 ± 1†† | ND |
| PEA | 2650 ± 151** | 9455 ± 806** | 564 ± 38†† | 441 ± 35†† | 312 ± 11† |
| OEA | 1543 ± 136** | 6402 ± 236** | 239 ± 12†† | 137 ± 7†† | 204 ± 19† |
| FAAH-NS | |||||
| Anandamide | 11 ± 3 | 4.8 ± 0.5 | 109 ± 12†† | 9.4 ± 1.2†† | ND |
| PEA | 230 ± 44 | 1412 ± 80 | 525 ± 23†† | 285 ± 52† | 376 ± 41† |
| OEA | 88 ± 11 | 543 ± 56 | 224 ± 6†† | 105 ± 13†† | 231 ± 31† |
PEA, N-palmitoyl ethanolamine; OEA, N-oleoyl ethanolamine. **, P < 0.01 for FAAH-/- mice vs. FAAH+/- mice or FAAH-NS mice; †, P < 0.05, ††, P ≤ 0.01 for FAAH-/- mice or FAAH-NS mice vs. FAAH+/- mice. ND, not determined (kidney samples possessed an abundant contaminating metabolite of equivalent mass to the sodium adduct of anandamide that obscured detection of this FAA). Data are reported as means ± SE. n = 3-6 mice per group.