Figure 4.
Root Growth Is Strongly Retarded in the ahk2-1 ahk3-1 ahk4-1 Triple Mutant.
(A) The kinetics of root growth. Plants were germinated and grown on the surface of MS agar plates with continuous fluorescent illumination at 22°C. The length of main roots was measured every day. Each value represents the average with standard deviation for 20 plants. The strains used were as follows: Col (open circles), Ws (closed circles), ahk2-1 ahk4-1 (open triangles), ahk3-1 ahk4-1 (closed triangles), ahk2-1 ahk3-1 (open squares), and ahk2-1 ahk3-1 ahk4-1 (closed squares).
(B) Longitudinal section of the hypocotyl of the ahk2-1 ahk3-1 ahk4-1 triple mutant (12-d-old seedling). The main root and adventitious roots are indicated by an arrow and arrowheads, respectively.
(C) to (F) Propidium iodine–stained root tips of 5-d-old seedlings of Col (C), Ws (D), ahk2-1 ahk3-1 (E), and ahk2-1 ahk3-1 ahk4-1 (F) plants. Bars = 50 μm.
(G) to (J) DAPI-stained root tips of 5-d-old seedlings of Col (G), Ws (H), ahk2-1 ahk3-1 (I), and ahk2-1 ahk3-1 ahk4-1 (J) plants. Bars = 50 μm.