FIG. 1.
(A) Schematic diagram showing restriction sites and ccpA gene orientation in the 3.8-kb HindIII insert in pSM310. (B) Diagram showing the strategy used for allele replacement of the wild-type copy of the ccpA gene of C. perfringens with an insertionally inactivated copy. The location of the ccpA-specific probe is shown beneath the N-terminal coding region of the ccpA gene.