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. 2004 Aug;186(16):5221–5229. doi: 10.1128/JB.186.16.5221-5229.2004

FIG. 1.

FIG. 1.

(A) Schematic diagram showing restriction sites and ccpA gene orientation in the 3.8-kb HindIII insert in pSM310. (B) Diagram showing the strategy used for allele replacement of the wild-type copy of the ccpA gene of C. perfringens with an insertionally inactivated copy. The location of the ccpA-specific probe is shown beneath the N-terminal coding region of the ccpA gene.