Figure 2. TC odor responses are sensitized by raphe inputs.
a) 2-photon image showing resting fluorescence of GCaMP6s in TCs.
b) Time course of florescence responses of two TCs to raphe stimulation (black), odor stimulation (light green) and raphe and odor stimulation (purple). Bars denote odor timing (light green) and raphe stimulation timing (orange). Error bars are SEM.
c) Time course of responses to 6 odors in 62 TCs in the imaged region shown in (a). Light green and orange bars at bottom indicate the timing of the odor and raphe stimulation, respectively.
d) Heat map representing the double rank ordered odor responses without (light green) and with (purple) raphe activation. Each row indicates the odor response amplitudes of single cells, with the rows themselves rank ordered in increasing amplitude of responses (summed across all odors). The order of cells was determined in the left panel and was maintained for the right panel (with raphe stimulation) for direct comparison. Bar plots on the right show the normalized summed activity of single cells and bar plots at the bottom show normalized summed activity for odors.
e) Bar plot (top) showing the change in the number of odors that elicited responses without and with raphe stimulation for cells shown in c (372 cell-odor pairs, p = 7.50×10−13, Wilcoxon signed-rank). Bar plot (bottom) showing the change in the number of odors that elicited responses without and with raphe stimulation for all cell odor pairs from 6 animals (1087 cell-odor pairs, p = 1.68×10−10, Wilcoxon signed-rank).
f) Cumulative distribution of the change in florescence for TCs without raphe stimulation (light green) or with raphe stimulation (purple) for all the cell odor pairs shown in c and d (top). Same for TCs without (black) or with raphe stimulation (purple) for all the cell odor pairs (n=1087) across 6 animals (bottom).
g) Scatter plots showing change in odor response for each TC when the raphe is stimulated (compared to odor response on its own), as a function of raphe response (left) and odor response (right) for that TC. Each dot is a single cell odor pair.
h) Three dimensional scatter plot showing the responses for TCs (372 odor cell pairs) for odor and raphe stimulation. The prediction surface, shown as a grid, is from the interaction based regression model (see Methods).
i) Scatter plot showing the predicted based on interactive model vs observed responses for odor and raphe stimulation (colors depict different odors, R2 = 0.88).
j)Scatter plot showing the predicted vs observed responses for odor and raphe stimulation (colors depict different animals, R2 = 0.86 for n=6 animals, 1087 cell-odor pairs, p =1.29× 10−21, F test).