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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Surg Res. 2016 Apr 22;204(1):47–54. doi: 10.1016/j.jss.2016.04.021

Figure 3. Inhibition of AHR mRNA expression abrogates AHR activity induced by indole-3-carbinol (I3C).

Figure 3

Si-scramble cells display a more robust activation AHR signaling (in the form of CYP1A1 mRNA expression) than si-AHR cells following I3C treatment. Cells were treated with either 500 μM I3C or 0.01% DMSO for 24 hours. Wild type HCT116 mRNA response is depicted as an additional model. Data are presented as relative fold induction of CYP1A1 mRNA of I3C treated cells over DMSO treated cells (controlled to internal levels of β-actin) ± standard error (representing at least three discrete experiments). “*” indicates statistical significance between I3C treated mRNA levels and vehicle (DMSO) controls in all cell types (p<0.05). “**” with brackets indicate statistical significance of I3C treated mRNA level differences between si-scramble and si-AHR cells (p<0.05).