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. 1991 Oct;44(10):844–848. doi: 10.1136/jcp.44.10.844

Detection of Epstein-Barr virus genomes in Hodgkin's disease: relation to age.

R F Jarrett 1, A Gallagher 1, D B Jones 1, F E Alexander 1, A S Krajewski 1, A Kelsey 1, J Adams 1, B Angus 1, S Gledhill 1, D H Wright 1, et al.
PMCID: PMC496672  PMID: 1660054

Abstract

An investigation as to whether any particular subgroup of patients with Hodgkin's disease was particularly likely to be Epstein-Barr virus (EBV) genome positive was made on samples from 95 patients. These were grouped according to age and Hodgkin's disease subtype, and analysed using Southern blot analysis. Most samples from children or adults aged 50 years or over contained detectable EBV genomes; samples from young adults were only rarely positive. The differences in EBV positivity by age were highly significant, but there was no significant association between EBV and histological subtype after allowing for the effect of age. The results support the hypothesis that Hodgkin's disease in different age groups may have different aetiologies, and suggest that EBV does have a pathogenetic role in Hodgkin's disease in children and older age groups.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander F. E., Williams J., McKinney P. A., Ricketts T. J., Cartwright R. A. A specialist leukaemia/lymphoma registry in the UK. Part 2: Clustering of Hodgkin's disease. Br J Cancer. 1989 Dec;60(6):948–952. doi: 10.1038/bjc.1989.396. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anagnostopoulos I., Herbst H., Niedobitek G., Stein H. Demonstration of monoclonal EBV genomes in Hodgkin's disease and Ki-1-positive anaplastic large cell lymphoma by combined Southern blot and in situ hybridization. Blood. 1989 Aug 1;74(2):810–816. [PubMed] [Google Scholar]
  3. Andiman W., Gradoville L., Heston L., Neydorff R., Savage M. E., Kitchingman G., Shedd D., Miller G. Use of cloned probes to detect Epstein-Barr viral DNA in tissues of patients with neoplastic and lymphoproliferative diseases. J Infect Dis. 1983 Dec;148(6):967–977. doi: 10.1093/infdis/148.6.967. [DOI] [PubMed] [Google Scholar]
  4. Arrand J. R., Rymo L., Walsh J. E., Björck E., Lindahl T., Griffin B. E. Molecular cloning of the complete Epstein-Barr virus genome as a set of overlapping restriction endonuclease fragments. Nucleic Acids Res. 1981 Jul 10;9(13):2999–3014. doi: 10.1093/nar/9.13.2999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bignon Y. J., Bernard D., Curé H., Fonck Y., Pauchard J., Travade P., Legros M., Dastugue B., Plagne R. Detection of Epstein-Barr viral genomes in lymph nodes of Hodgkin's disease patients. Mol Carcinog. 1990;3(1):9–11. doi: 10.1002/mc.2940030105. [DOI] [PubMed] [Google Scholar]
  6. Boiocchi M., Carbone A., De Re V., Dolcetti R. Is the Epstein-Barr virus involved in Hodgkin's disease? Tumori. 1989 Aug 31;75(4):345–350. doi: 10.1177/030089168907500409. [DOI] [PubMed] [Google Scholar]
  7. Evans A. S., Gutensohn N. M. A population-based case-control study of EBV and other viral antibodies among persons with Hodgkin's disease and their siblings. Int J Cancer. 1984 Aug 15;34(2):149–157. doi: 10.1002/ijc.2910340203. [DOI] [PubMed] [Google Scholar]
  8. Frizzera G. The clinico-pathological expressions of Epstein-Barr virus infection in lymphoid tissues. Virchows Arch B Cell Pathol Incl Mol Pathol. 1987;53(1):1–12. doi: 10.1007/BF02890218. [DOI] [PubMed] [Google Scholar]
  9. Gobbi P. G., Cavalli C., Federico M., Bertoloni D., Di Prisco U. A., Rossi A., Silingardi V., Mauri C., Ascari E. Hodgkin's disease prognosis: a directly predictive equation. Lancet. 1988 Mar 26;1(8587):675–679. doi: 10.1016/s0140-6736(88)91477-8. [DOI] [PubMed] [Google Scholar]
  10. Gutensohn N. M. Social class and age at diagnosis of Hodgkin's disease: new epidemiologic evidence for the "two-disease hypothesis". Cancer Treat Rep. 1982 Apr;66(4):689–695. [PubMed] [Google Scholar]
  11. Hamilton-Dutoit S. J., Pallesen G., Karkov J., Skinhøj P., Franzmann M. B., Pedersen C. Identification of EBV-DNA in tumour cells of AIDS-related lymphomas by in-situ hybridisation. Lancet. 1989 Mar 11;1(8637):554–552. doi: 10.1016/s0140-6736(89)90093-7. [DOI] [PubMed] [Google Scholar]
  12. Henle W., Henle G. Epstein-Barr virus-related serology in Hodgkin's disease. Natl Cancer Inst Monogr. 1973 May;36:79–84. [PubMed] [Google Scholar]
  13. Herbst H., Niedobitek G., Kneba M., Hummel M., Finn T., Anagnostopoulos I., Bergholz M., Krieger G., Stein H. High incidence of Epstein-Barr virus genomes in Hodgkin's disease. Am J Pathol. 1990 Jul;137(1):13–18. [PMC free article] [PubMed] [Google Scholar]
  14. Herbst H., Tippelmann G., Anagnostopoulos I., Gerdes J., Schwarting R., Boehm T., Pileri S., Jones D. B., Stein H. Immunoglobulin and T-cell receptor gene rearrangements in Hodgkin's disease and Ki-1-positive anaplastic large cell lymphoma: dissociation between phenotype and genotype. Leuk Res. 1989;13(2):103–116. doi: 10.1016/0145-2126(89)90134-3. [DOI] [PubMed] [Google Scholar]
  15. Hesse J., Levine P. H., Ebbesen P., Connelly R. R., Mordhorst C. H. A case control study on immunity to two Epstein-Barr virus-associated antigens, and to herpes simplex virus and adenovirus in a population-based group of patients with Hodgkin's disease in Denmark, 1971-73. Int J Cancer. 1977 Jan;19(1):49–58. doi: 10.1002/ijc.2910190108. [DOI] [PubMed] [Google Scholar]
  16. Jarrett R. F., Gledhill S., Qureshi F., Crae S. H., Madhok R., Brown I., Evans I., Krajewski A., O'Brien C. J., Cartwright R. A. Identification of human herpesvirus 6-specific DNA sequences in two patients with non-Hodgkin's lymphoma. Leukemia. 1988 Aug;2(8):496–502. [PubMed] [Google Scholar]
  17. Libetta C. M., Pringle J. H., Angel C. A., Craft A. W., Malcolm A. J., Lauder I. Demonstration of Epstein-Barr viral DNA in formalin-fixed, paraffin-embedded samples of Hodgkin's disease. J Pathol. 1990 Jul;161(3):255–260. doi: 10.1002/path.1711610313. [DOI] [PubMed] [Google Scholar]
  18. Mueller N., Evans A., Harris N. L., Comstock G. W., Jellum E., Magnus K., Orentreich N., Polk B. F., Vogelman J. Hodgkin's disease and Epstein-Barr virus. Altered antibody pattern before diagnosis. N Engl J Med. 1989 Mar 16;320(11):689–695. doi: 10.1056/NEJM198903163201103. [DOI] [PubMed] [Google Scholar]
  19. Nalesnik M. A., Jaffe R., Starzl T. E., Demetris A. J., Porter K., Burnham J. A., Makowka L., Ho M., Locker J. The pathology of posttransplant lymphoproliferative disorders occurring in the setting of cyclosporine A-prednisone immunosuppression. Am J Pathol. 1988 Oct;133(1):173–192. [PMC free article] [PubMed] [Google Scholar]
  20. Pallesen G., Hamilton-Dutoit S. J., Rowe M., Young L. S. Expression of Epstein-Barr virus latent gene products in tumour cells of Hodgkin's disease. Lancet. 1991 Feb 9;337(8737):320–322. doi: 10.1016/0140-6736(91)90943-j. [DOI] [PubMed] [Google Scholar]
  21. Raab-Traub N., Flynn K. The structure of the termini of the Epstein-Barr virus as a marker of clonal cellular proliferation. Cell. 1986 Dec 26;47(6):883–889. doi: 10.1016/0092-8674(86)90803-2. [DOI] [PubMed] [Google Scholar]
  22. Reif A. E., Allen J. M. The fragility of normal and leukemic lymphocytes of AKR mice: a study of cell injury by physical agents. Cancer Res. 1966 Jan;26(1):131–136. [PubMed] [Google Scholar]
  23. Sample J., Young L., Martin B., Chatman T., Kieff E., Rickinson A., Kieff E. Epstein-Barr virus types 1 and 2 differ in their EBNA-3A, EBNA-3B, and EBNA-3C genes. J Virol. 1990 Sep;64(9):4084–4092. doi: 10.1128/jvi.64.9.4084-4092.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Serrano M., Bellas C., Campo E., Ribera J., Martín C., Rubio R., Ruiz C., Ocaña I., Buzón L., Yebra M. Hodgkin's disease in patients with antibodies to human immunodeficiency virus. A study of 22 patients. Cancer. 1990 May 15;65(10):2248–2254. doi: 10.1002/1097-0142(19900515)65:10<2248::aid-cncr2820651015>3.0.co;2-w. [DOI] [PubMed] [Google Scholar]
  25. Sixbey J. W., Shirley P., Chesney P. J., Buntin D. M., Resnick L. Detection of a second widespread strain of Epstein-Barr virus. Lancet. 1989 Sep 30;2(8666):761–765. doi: 10.1016/s0140-6736(89)90829-5. [DOI] [PubMed] [Google Scholar]
  26. Staal S. P., Ambinder R., Beschorner W. E., Hayward G. S., Mann R. A survey of Epstein-Barr virus DNA in lymphoid tissue. Frequent detection in Hodgkin's disease. Am J Clin Pathol. 1989 Jan;91(1):1–5. doi: 10.1093/ajcp/91.1.1. [DOI] [PubMed] [Google Scholar]
  27. Sternås L., Middleton T., Sugden B. The average number of molecules of Epstein-Barr nuclear antigen 1 per cell does not correlate with the average number of Epstein-Barr virus (EBV) DNA molecules per cell among different clones of EBV-immortalized cells. J Virol. 1990 May;64(5):2407–2410. doi: 10.1128/jvi.64.5.2407-2410.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Uccini S., Monardo F., Ruco L. P., Baroni C. D., Faggioni A., Agliano A. M., Gradilone A., Manzari V., Vago L., Costanzi G. High frequency of Epstein-Barr virus genome in HIV-positive patients with Hodgkin's disease. Lancet. 1989 Jun 24;1(8652):1458–1458. doi: 10.1016/s0140-6736(89)90171-2. [DOI] [PubMed] [Google Scholar]
  29. Uccini S., Monardo F., Stoppacciaro A., Gradilone A., Aglianò A. M., Faggioni A., Manzari V., Vago L., Costanzi G., Ruco L. P. High frequency of Epstein-Barr virus genome detection in Hodgkin's disease of HIV-positive patients. Int J Cancer. 1990 Oct 15;46(4):581–585. doi: 10.1002/ijc.2910460405. [DOI] [PubMed] [Google Scholar]
  30. Walker A., Schoenfeld E. R., Lowman J. T., Mettlin C. J., MacMillan J., Grufferman S. Survival of the older patient compared with the younger patient with Hodgkin's disease. Influence of histologic type, staging, and treatment. Cancer. 1990 Apr 1;65(7):1635–1640. doi: 10.1002/1097-0142(19900401)65:7<1635::aid-cncr2820650730>3.0.co;2-m. [DOI] [PubMed] [Google Scholar]
  31. Weiss L. M., Movahed L. A., Warnke R. A., Sklar J. Detection of Epstein-Barr viral genomes in Reed-Sternberg cells of Hodgkin's disease. N Engl J Med. 1989 Feb 23;320(8):502–506. doi: 10.1056/NEJM198902233200806. [DOI] [PubMed] [Google Scholar]
  32. Weiss L. M., Strickler J. G., Warnke R. A., Purtilo D. T., Sklar J. Epstein-Barr viral DNA in tissues of Hodgkin's disease. Am J Pathol. 1987 Oct;129(1):86–91. [PMC free article] [PubMed] [Google Scholar]
  33. Wu T. C., Mann R. B., Charache P., Hayward S. D., Staal S., Lambe B. C., Ambinder R. F. Detection of EBV gene expression in Reed-Sternberg cells of Hodgkin's disease. Int J Cancer. 1990 Nov 15;46(5):801–804. doi: 10.1002/ijc.2910460509. [DOI] [PubMed] [Google Scholar]
  34. Young L. S., Yao Q. Y., Rooney C. M., Sculley T. B., Moss D. J., Rupani H., Laux G., Bornkamm G. W., Rickinson A. B. New type B isolates of Epstein-Barr virus from Burkitt's lymphoma and from normal individuals in endemic areas. J Gen Virol. 1987 Nov;68(Pt 11):2853–2862. doi: 10.1099/0022-1317-68-11-2853. [DOI] [PubMed] [Google Scholar]
  35. Zutter M. M., Martin P. J., Sale G. E., Shulman H. M., Fisher L., Thomas E. D., Durnam D. M. Epstein-Barr virus lymphoproliferation after bone marrow transplantation. Blood. 1988 Aug;72(2):520–529. [PubMed] [Google Scholar]

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