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. Author manuscript; available in PMC: 2017 Aug 11.
Published in final edited form as: Cell. 2016 Jul 21;166(4):841–854. doi: 10.1016/j.cell.2016.06.040

Figure 7. Perinatal requirement of IL-33 in licensing of uncoupled respiration.

Figure 7

(A) Immunoblotting for UCP1 protein in BAT during the perinatal period in WT and Il33−/− mice housed at 22°C (n=2 per genotype and time point). (B) Immunoblotting for UCP1 protein in P0.5 BAT of WT and Il1rl1−/− mice housed at 22°C (n=3 per genotype). (C) Immunoblotting for components of mitochondrial respiratory chain and Cytochrome C in purified mitochondria from BAT of P0.5 WT, Il33−/−, and Ucp1−/− mice (n=2 per genotype). (D, E) Quantification of activities of complex I (D) and complex IV (E) in purified mitochondria from P0.5 BAT of WT, Il33−/−, and Ucp1−/− mice (n=3 per genotype). (F-H) Representative transmission electron micrographs of P0.5 BAT of WT, Il33−/−, and Ucp1−/− mice, magnification 4000x. Data are represented as mean ± SEM.