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. 2016 Nov 7;6:36551. doi: 10.1038/srep36551

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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IL-17, IL-6, IL-8, VEGF mRNA and protein and CD31 protein levels were determined in human lung adenocarcinoma tissues by qRT-PCR or IHC, respectively. (A) Spearman’s correlation analysis was performed to analyse the correlation between IL-17 (a), IL-6 (b), IL-8 (c), VEGF (d) protein expression and tumour microvessel density (MVD) by CD31 staining in tumor tissues with human lung adenocarcinoma. (B) Spearman’s correlation analysis was performed to analyse the correlation between IL-17 (a), IL-6 (b), IL-8 (c), VEGF (d) expression and tumour microvessel density by CD31 staining in 28 tissues with human lung adenocarcinoma. mRNA expression levels were calculated using the −ΔΔCt method, and target gene expression was normalized to the GAPDH housekeeping gene.