Fig. 1.
Analysis of primary cell lines for smooth muscle cell markers. A: immunoblot analysis performed on whole cell protein lysates from shunt and control primary PASMC lines as well as control, non-smooth muscle cell lines with primary antibodies specifically targeting α smooth muscle actin (from mouse) and NH2-terminal actin (from rabbit), followed by incubation with anti-mouse and anti-rabbit secondary antibodies conjugated to red and green fluorophores, respectively. B: the smooth muscle lineage of each cell line was confirmed by immunofluorescence staining with anti-Calponin. All isolated cell lines from control and shunt animals express the cell lineage typical marker.