Fig. 5.
Targeted metabolite analysis in shunt vs. control primary PASMCs. Selected metabolites were measured in shunt and control PASMCs using a targeted approach with tandem HPLC/QTOF mass spectrometry. Metabolites were quantified by comparison with 13C labeled internal standards included at known concentration during the experimental extraction and analysis procedures. Results are normalized to total protein content of the sample with concentrations of metabolites expressed in picomoles/microgram of protein. Error bars indicate 1 SD. *Significance by Student's t-test with P value < 0.05. A: targeted analysis of intermediates from the glycolytic pathway including fructose-1,6-bisphosphate, phosphoenolpyruvate and the ratio of fructose-6-phosphate to glucose-6-phosphate. B: targeted analysis of intermediates from the pentose phosphate pathway including d-sedoheptulose-7-phosphate, erythritol and the ratio of ribose-5-phosphate to xylulose-5-phosphate. C: comparison of acetyl-CoA levels in shunt and control PASMCs. D: targeted analysis of intermediate metabolites from the TCA cycle including succinate, malate, and the ratio of citrate to isocitrate.