Figure 3.
TIP27 interacts specifically with the nuclear orphan receptor TAK1. CV-1 cells grown in DMEM containing charcoal-stripped FBS were transfected with (UAS)5-LUC, pRL-SV40, pM or pM-TIP27, and either VP16, VP16-TAK1, VP16-AR, VP16-ERα, VP16-PR-A, VP16-LXRα, VP16-PPARγ, VP16-RXRα, VP16-RTR or VP16-RORγ expression vectors as indicated. After 24 h, cells were treated with the respective ligand [1 μM 17β-estradiol (ERα), 22R-hydroxycholesterol (LXRα), GW347845 (PPARγ), SRI 11246 (RXRα), 5α-dihydrotestosterone (AR) or 100 nM progesterone (PR-A)] or vehicle and 48 h later assayed for reporter activities as described in Materials and Methods. The relative firefly LUC reporter activity was calculated and plotted as stated in Materials and Methods.