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Regulation of human cTNT by MBNL1 is independent of CELF regulation. The (A) wild-type cTNT minigene or a (B) mutant cTNT minigene with point mutations that prevent CUG-BP1 binding and regulation were cotransfected with the indicated siRNA constructs, a plasmid expressing a DMPK minigene with 960 CUG repeats (Philips et al, 1998) or a GFP–MBNL1 expression plasmid in HeLa cells.
