TABLE 2.
PCR results in 66 vascular specimens by different PCR assays
| No. of specimens | Result ofa:
|
||||
|---|---|---|---|---|---|
| Single-step PCR
|
Nested PCR
|
PCR-based RLB C | |||
| A1 | A2b | B1b | B2b | ||
| 18 | − | − | − | − | − |
| 28 | PCR+ | − | − | − | − |
| 8 | PCR+ | − | − | − | Cspp+ |
| 12 | − | − | − | − | Cspp+ |
a
In assays A1 and B1, Taq polymerase was used; in assays A2 and B2, AmpliTaq Gold polymerase was used; in assay C, Hotstar Taq DNA polymerase was used. −, PCR was negative both in agarose gel electrophoresis and after hybridization; PCR+, PCR was positive in agarose gel electrophoresis and negative after hybridization; Cspp+, Chlamydia sp. positive in RLB hybridization. A total of 36 specimens were positive by single-step PCR assay A1, and a total of 20 specimens were positive by PCR-based RLB assay C.
b
None of the specimens was C. pneumoniae positive.