Fig. 3.
Confirmation of C/EBPβ targets identified by orthogonal analysis. (A) Conventional ChIP of C/EBPβ target genes. Chromatin was prepared from quiescent wild-type or C/EBPβ–/– mutant mouse livers and immunoprecipitated by using C/EBPβ or nonimmune antibody. The immunoprecipitates and input chromatin were subjected to PCR by using primers specific for the indicated genes. Typical results from experiments performed in duplicate are presented. (B) Expression of C/EBPβ targets in quiescent and regenerating liver. Quantitative real-time PCR was performed by using reverse-transcribed RNA obtained from wild-type and C/EBPβ–/– mutant mouse livers before and at 2, 16, 24, 40, and 48 h after partial hepatectomy. The expression of each gene is presented as a ratio to the expression of TATA binding protein. Error bars indicate standard error of the mean. *, P ≤ 0.05.