Table 2. Infection and dissemination of Asian lineage ZIKV strain FSS13025 (Cambodia, 2010) in A. aegypti.
Strain | Blood meal titer (Log10 FFU/ml)a | Infection rate(%)b | Disseminated infection rate (%)c | Dissemination rate(%)d |
---|---|---|---|---|
FSS13025 parental | 6.2 | 18/42 (43) | 11/42 (26) | 11/18 (61) |
FSS13025 recombinant | 6.5 | 33/42 (78) | 19/42 (45) | 19/33 (58) |
After blood meal, viral titers for both parental and recombinant viruses were measured by focus forming assay to ensure the accuracy of virus amounts in the blood meal.
Infection rate = Number of infected mosquitos / number of engorged mosquitos × 100%
Disseminated infection rate = Number of disseminated mosquitos / number of engorged mosquitos × 100%
Dissemination rate (%) = Number of disseminated mosquitos / number of infected mosquitos × 100%