Fig 4.
Analysis of myogenic markers in mutant and normal mice. Semiquantitative RT-PCR analysis of myosin heavy chains (embryonic, MHCe; perinatal, MHCp; type I, MHCI; type IIa, MHCIIa; type IIb, MHCIIb; type IIx, MHCIIx), desmin and vimentin transcripts from hindlimb skeletal muscle (supero-posterior compartment, SPC) of wild-type (WT) and KO mice at postnatal day 15 (i) and at 7 weeks of age (phase III, ii). Exon 4 deletion in Mtm1 transcript of KO muscle leads to a smaller PCR product. As negative control (−), the reverse transcriptase was omitted from the sample (n = 2).