Table 1. Summary of alanine-scan mutation data classified by functional group of substituted residues.
| Class | Amino acid | Complete loss | Partial loss | Neutral |
|---|---|---|---|---|
| Acidic | D | 0 | 3 | 0 |
| E | 2 | 1 | 4 | |
| Basic | H | 0 | 0 | 0 |
| K | 4 | 1 | 5 | |
| R | 2 | 2 | 2 | |
| Uncharged polar | N | 2 | 5 | 2 |
| C | 0 | 0 | 1 | |
| S | 0 | 1 | 1 | |
| T | 0 | 2 | 3 | |
| Y | 2 | 1 | 2 | |
| Q | 1 | 0 | 3 | |
| Nonpolar | F | 2 | 0 | 1 |
| G | 0 | 1 | 0 | |
| I | 1 | 1 | 2 | |
| L | 2 | 1 | 4 | |
| P | 0 | 0 | 1 | |
| V | 0 | 3 | 4 | |
| W | 2 | 0 | 0 |
Residues that affected binding function of DBPII were distributed across each category of biochemical character. The sums of complete and partial loss of function and of neutral function were 20, 22, and 35, respectively.