Figure 4. Radiolytic footprinting of ACKR3 complexes.
(a) All detectable ACKR3 oxidation rates mapped onto the sequence. Rates are shown as the log of RateACKR3:CCX777/RateACKR3:CXCL12. Residues are coloured using a gradient where blue corresponds to reduced oxidation rates in the ACKR3:CCX777 complex (protection by CCX777) and red corresponds to reduced oxidation rates in the ACKR3:CXCL12 complex. (b) Radiolytic footprinting results mapped onto a topology plot of ACKR3. Residues for which oxidation rates were measured are highlighted and coloured according to the scheme in (a). Residues where multiple rates were measured are coloured by the average log (RateACKR3:CCX777/RateACKR3:CXCL12 ) of the multiple oxidation events. Half-coloured circles signify that an oxidation rate was mapped to one or both of two neighbouring residues.