Table S2.
Primers used to clone the Zika genes into the pYZ3N fission yeast expression vector for the construction of GFP-fusion proteins
| Gene name | Size, bp | 5′ primer | 3′ primer | Cloning sites |
| anaC | 366 | CTGTC GCGGCCGC T AAAAACCCAAAGAAGAAATCCG | CTCG GGATCC TTA TGCCATGGCTGTAGTCAGCAG | NotI/BamHI |
| C | 312 | CTGTC GCGGCCGC T AAAAACCCAAAGAAGAAATCCG | CTCG GGATCC TTA ACGTCTCTTCCTCTCTTTCCTAGC | NotI/BamHI |
| Pr | 279 | CTGTC GCGGCCGC A GCAGAGATCACTAGACGCGGG | CTCG GGATCC TTA TCTTCTAGATCGCCGTGCCTC | NotI/BamHI |
| M | 225 | CTGTC GCGGCCGC A GCCGTGACGCTCCCTTCTC | CTCG GGATCC TTA ACTGTATGCCGGGGCAATC | NotI/BamHI |
| prM | 504 | CTGTC GCGGCCGC A GCAGAGATCACTAGACGCGGG | CTCG GGATCC TTA ACTGTATGCCGGGGCAATC | NotI/BamHI |
| E | 1,512 | CTGTC GCGGCCGC A ATCAGGTGCATTGGAGTCAGC | CTCG GGATCC TTA AGCAGAAACAGCCGTGGAGAG | NotI/BamHI |
| 2K | 69 | CTGTC GCGGCCGC A TCTCCCCAAGATAACCAGATGG | CTCG GGATCC TTA TGCAGTTATCAAACCTAGAAGGC | NotI/BamHI |
| NS1 | 1,056 | CTGTC GCGGCCGC A GACGTGGGGTGCTCAGTGGAC | CTCG GGATCC TTA CGCTGTCACCATTGACCTCAC | NotI/BamHI |
| NS2A | 678 | CTGTC GCGGCCGC A GGGTCAACCGATCATATGGAC | CTCG GGATCC TTA CCGCTTCCCACTCCTTGTG | NotI/BamHI |
| NS2B | 390 | CTGTC GCGGCCGC A AGCTGGCCCCCTAGTGAAG | CTCG GGATCC TTA CCTTTTCCCAGTCTTCACATACAC | NotI/BamHI |
| NS3 | 1,851 | CTGTC GCGGCCGC A AGTGGCGCCCTCTGGGAC | CTCG GGATCC TTA TCTTTTTCCAGCGGCGAATT | NotI/BamHI |
| NS4A | 381 | CTGTC GCGGCCGC A GGAGCGGCTTTGGGAGTAAT | CTCG GGATCC TTA TCTTTGCTTCTCTGGCTCGG | NotI/BamHI |
| NS4B | 753 | CTGTC GCGGCCGC T AACGAACTTGGATGGCTGG | CTCG GGATCC TTA ACGTCTCTTAACCAGGCCAG | NotI/BamHI |
| NS5 | 2,709 | CTGTC GCGGCCGC A GGAGGTGGGACGGGAGAGAC | CTCG GTCGAC TTA CAACACTCCGGGTGTGGACC | NotI/SalI |
Restriction enzymatic sites are underlined.