Fig E9.
Expression of scaffold proteins in Treg cells. A, Molecular interactions between PTEN and PHLPP1/2 suggest a protein network that links those phosphatases with the structural scaffold proteins NHERF1/2 and the membrane linker ezrin (EZR; String database 9.0, high-confidence setting). B, mRNA gene expression analysis in PBMCs compared with FACS-sorted B cells, whole CD4+ T cells, Tmem cells, and CD4+CD25+/high Treg cells. Comparative gene expression analysis of the structural proteins NHERF1, EZR, DLG1, and moesin (MSN) is shown. Values are normalized to the 18S rRNA level in each cell population. Means and SDs are shown for triplicate values. NHERF2 expression was not detected (data not shown). C, Immunohistochemical detection of NHERF1 expression in FOXP3+ cells. Images show representative NHERF1 staining in the control appendix section. DAPI, 4′, 6′-Diamidino-2-phenylindole.