Fig. 2.
Homozygous KO of SREBP2 from astrocytes impairs brain development. hGFAP-cre;Srebf2lox/lox mice (designated throughout as G-SB2KO) were compared with littermate controls. (A and B) Brains of G-SB2KO mice weighed less than controls. n = 6 per group. (C) Consecutive 1-mm slices through fresh brain. (D) Expression of Srebf2 measured by quantitative PCR (qPCR) from dissected brain regions, cortex, amygdala (Amyg), hypothalamus (Hypo), and hippocampus (Hippo). n = 5 control, 4 G-SB2KO. (E) Immunostaining for GFAP (red), SREBP2 (green), DAPI (blue), and colocalization (yellow) in corpus callosum and thalamus. Representative 40× confocal images of n = 3 control, 2 G-SB2KO. Student’s t test *P < 0.05, ***P < 0.001 compared with control.